Exploring fermentation strategies for enhanced lactic acid production with polyvinyl alcohol-immobilized Lactobacillus plantarum 23 using microalgae as feedstock

•Lactic acid (LA) fermentation allows green production of lactic acid.•PVA-immobilization of L. plantarum enhanced LA titer and yield by 40%.•Continuous fermentation with glucose gave the highest productivity of 14.2 g/L/h.•In situ lactic acid removal during fermentation improved LA productivity &am...

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Bibliographic Details
Published in:Bioresource technology 2020-07, Vol.308, p.123266-123266, Article 123266
Main Authors: Chen, Po-Ting, Hong, Zih-Syuan, Cheng, Chieh-Lun, Ng, I-Son, Lo, Yung-Chung, Nagarajan, Dillirani, Chang, Jo-Shu
Format: Article
Language:English
Subjects:
Chlorella vulgaris
Fermentation
Fermentation strategy
Immobilized cells
Lactic Acid
Lactobacillus plantarum
Microalgae
Polyvinyl Alcohol
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Summary:•Lactic acid (LA) fermentation allows green production of lactic acid.•PVA-immobilization of L. plantarum enhanced LA titer and yield by 40%.•Continuous fermentation with glucose gave the highest productivity of 14.2 g/L/h.•In situ lactic acid removal during fermentation improved LA productivity & yield.•LA productivity reached 9.93 g/L/h when using microalgal hydrolysate as substrate. Lactic acid (LA) fermentation was conducted with suspended and immobilized cells of an isolated Lactobacillus plantarum 23 strain using various fermentation strategies. Glucose and an alternative, relatively inexpensive carbon source - the hydrolysate of microalga Chlorella vulgaris ESP-31, were used as the carbon source. Batch fermentation using immobilized cells of L. plantarum 23 could enhance LA titer and yield by 43% and 39%, respectively, when compared with the suspended culture. Fed-batch culture integrated with in situ LA removal via ion exchange raised LA productivity by 72% by overcoming product inhibition. The highest LA productivity from glucose with PVA immobilized cells was 14.22 g/L/h, achieved under continuous operation at 50% w/v loading of immobilized beads and hydraulic retention time (HRT) of 2 h. PVA immobilized L. plantarum 23 could also use microalgal hydrolysate as the renewable carbon source, and the highest LA productivity was 9.93 g/L/h under continuous fermentation at 4 h HRT.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2020.123266