Ultra‐low depth sequencing of plasma cell DNA for the detection of copy number aberrations in multiple myeloma

Cytogenetic abnormalities are powerful prognostic factors in multiple myeloma (MM) and are routinely analyzed by FISH on bone marrow (BM) plasma cells (PC). Although considered the gold standard, FISH experiments can be laborious and expensive. Therefore, array‐CGH (aCGH) has been introduced as an a...

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Bibliographic Details
Published in:Genes chromosomes & cancer 2020-08, Vol.59 (8), p.465-471
Main Authors: Buedts, Lieselot, Smits, Sanne, Ameye, Geneviève, Lehnert, Stefan, Ding, Jia, Delforge, Michel, Vermeesch, Joris, Boeckx, Nancy, Tousseyn, Thomas, Michaux, Lucienne, Vandenberghe, Peter, Dewaele, Barbara
Format: Article
Language:English
Subjects:
Bone marrow
Copy number
Cytogenetics
Deoxyribonucleic acid
DNA
DNA sequencing
Genomes
molecular cytogenetics
Multiple myeloma
Next-generation sequencing
Plasma cells
prognostic factor
ultra‐low depth sequencing
Whole genome sequencing
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Summary:Cytogenetic abnormalities are powerful prognostic factors in multiple myeloma (MM) and are routinely analyzed by FISH on bone marrow (BM) plasma cells (PC). Although considered the gold standard, FISH experiments can be laborious and expensive. Therefore, array‐CGH (aCGH) has been introduced as an alternative approach for detecting copy number aberrations (CNA), reducing the number of FISH experiments per case and yielding genome‐wide information. Currently, next generation sequencing (NGS) technologies offer new perspectives for the diagnostic workup of malignant disorders. In this study, we examined ultra‐low depth whole genome sequencing (LDS) as a valid alternative for aCGH for the detection of CNA in BM PC in MM. To this end, BM aspirates obtained in a diagnostic setting from 20 MM cases were analyzed. CD138+ cell‐sorted samples were subjected to FISH analysis. DNA was extracted for subsequent aCGH and LDS analysis. CNA were detected by aCGH and LDS in all but one case. Importantly, all CNA identified by parallel first generation aCGH analysis were also detected by LDS, along with six additional CNA in five cases. One of these additional aberrations was in a region of prognostic importance in MM and was confirmed using FISH. However, risk stratification in these particular cases was unaffected. Thus, a perfectly concordant prognostication between array‐CGH and LDS was observed. This validates LDS as a novel and cost‐efficient tool for the detection of CNA in MM.
ISSN:1045-2257
1098-2264
DOI:10.1002/gcc.22848