Encapsulating chromogenic reaction substrates with porous hydrogel scaffolds onto arrayed capillary tubes toward a visual and high-throughput colorimetric strategy for rapid occult blood tests

A porous hydrogel scaffold was fabricated for the first time to encapsulate chromogenic reaction substrates onto arrayed capillary tubes, resulting in a visual and high-throughput colorimetric method for rapid occult blood tests (OBTs) based on the hemoglobin (Hgb)-catalyzed chromogenic reactions. G...

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Bibliographic Details
Published in:Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2017-02, Vol.5 (6), p.1159-1165
Main Authors: Jiang, Yao, Sun, Zongzhao, Zhang, Liyan, Qiao, Yuchun, Liu, Fengjuan, Cai, Yuanyuan, Zhang, Wenwen, Zhang, Qianqian, Duan, Zhiqiang, Wang, Hua
Format: Article
Language:English
Subjects:
Blood
Capillary tubes
Colorimetry
Diseases
Encapsulation
Hydrogels
Scaffolds
Substrates
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Summary:A porous hydrogel scaffold was fabricated for the first time to encapsulate chromogenic reaction substrates onto arrayed capillary tubes, resulting in a visual and high-throughput colorimetric method for rapid occult blood tests (OBTs) based on the hemoglobin (Hgb)-catalyzed chromogenic reactions. Gelatin (Gel), a biodegradable and biocompatible polymer, was introduced to couple with p -hydroxyphenyl-propionic acid (HPA) yielding the Gel-HPA hydrogel scaffold. Chromogenic reaction substrates of 3,3,5,5-tetramethylbenzidine and H 2 O 2 were then encapsulated into the Gel-HPA matrix and further attached onto the amine-derivatized capillary tubes by forming porous chromogenic composites through the HPA-mediated bridging of Gel by the oxidization of H 2 O 2 . The developed Hgb catalysis-based OBT platform can facilitate the detection of Hgb with the level down to 0.125 μg mL −1 in human excreta ( i.e. , saliva, urine, and feces) through capillarity-enabled automatic sampling. This simple, sensitive, selective, and high-throughput colorimetric method may be promising for the bedside OBT for point-of-care monitoring and rapid diagnostics of clinical bleeding diseases. A porous hydrogel composite was used to encapsulate the chromogenic reaction substrates onto capillary tubes arrayed for visual and high-throughput occult blood test.
ISSN:2050-750X
2050-7518
DOI:10.1039/c6tb02836e