Hsp27 reduces glycation‐induced toxicity and aggregation of alpha‐synuclein

• Published in: The FASEB journal 2020-05, Vol.34 (5), p.6718-6728
• Main Authors: Vicente Miranda, Hugo, Chegão, Ana, Oliveira, Márcia S., Fernandes Gomes, Bárbara, Enguita, Francisco J., Outeiro, Tiago Fleming
• Format: Article
• Language: English
• Subjects: alpha-Synuclein - chemistry; alpha-Synuclein - drug effects; alpha‐synuclein; Brain Neoplasms - drug therapy; Brain Neoplasms - genetics; Brain Neoplasms - metabolism; Brain Neoplasms - pathology; Glioma - drug therapy; Glioma - genetics; Glioma - metabolism; Glioma - pathology; glycation; Glycosylation; Heat-Shock Proteins - genetics; Heat-Shock Proteins - metabolism; Hsp27; Humans; Molecular Chaperones - genetics; Molecular Chaperones - metabolism; neurodegeneration; Parkinson's disease; Protein Aggregates - drug effects; Pyruvaldehyde - pharmacology; Tumor Cells, Cultured
• ISSN: 0892-6638; 1530-6860
• DOI: 10.1096/fj.201902936R

α‐synuclein (aSyn) is a major player in Parkinson's disease and a group of other disorders collectively known as synucleinopathies, but the precise molecular mechanisms involved are still unclear. aSyn, as virtually all proteins, undergoes a series of posttranslational modifications during its lifetime, which can affect its biology and pathobiology. We recently showed that glycation of aSyn by methylglyoxal (MGO) potentiates its oligomerization and toxicity, induces dopaminergic neuronal cell loss in mice, and affects motor performance in flies. Small heat‐shock proteins (sHsps) are molecular chaperones that facilitate the folding of proteins or target misfolded proteins for clearance. Importantly, sHsps were shown to prevent aSyn aggregation and cytotoxicity. Upon treating cells with increasing amounts of methylglyoxal, we found that the levels of Hsp27 decreased in a dose‐dependent manner. Therefore, we hypothesized that restoring the levels of Hsp27 in glycating environments could alleviate the pathogenicity of aSyn. Consistently, we found that Hsp27 reduced MGO‐induced aSyn aggregation in cells, leading to the formation of nontoxic aSyn species. Remarkably, increasing the levels of Hsp27 suppressed the deleterious effects induced by MGO. Our findings suggest that in glycating environments, the levels of Hsp27 are important for modulating the glycation‐associated cellular pathologies in synucleinopathies.