Carborane-β-cyclodextrin complexes as a supramolecular connector for bioactive surfaces

• Published in: Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2015-01, Vol.3 (4), p.539-545
• Main Authors: Neirynck, P, Schimer, J, Jonkheijm, P, Milroy, L-G, Cigler, P, Brunsveld, L
• Format: Article
• Language: English
• Subjects: Affinity; Biocompatibility; Cell adhesion; Connectors; Dynamical systems; Dynamics; Peptides; Strategy
• ISSN: 2050-750X; 2050-7518
• DOI: 10.1039/c4tb01489h

Supramolecular chemistry provides an attractive entry to generate dynamic and well-controlled bioactive surfaces. Novel host-guest systems are urgently needed to provide a broader affinity and applicability portfolio. A synthetic strategy to carborane-peptide bioconjugates was therefore developed to provide an entry to monovalent supramolecular functionalization of β-cyclodextrin coated surfaces. The β-cyclodextrin·carborane-cRGD surfaces are formed efficiently and with high affinity as demonstrated by IR-RAS, WCA, and QCM-D, compare favourable to existing bio-active host-guest surface assemblies, and display an efficient bioactivity, as illustrated by a strong functional effect of the supramolecular system on the cell adhesion and spreading properties. Cells seeded on the supramolecular surface displaying bioactive peptide epitopes exhibited a more elongated morphology, focal adhesions, and stronger cell adhesion compared to control surfaces. This highlights the macroscopic functionality of the novel supramolecular immobilization strategy.