Loading…
Protocol of proceedings with Fusobacterium nucleatum and optimization of ABTS method for detection of reactive oxygen species
Characterization of the ability of DSM 15643 and DSM 20482 strains in the presence of Cu and H O to reactive oxygen species generation. Spectrophotometric ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) method was used. Determination of: MIC for Cu , H O and ABTS; survivability of...
Saved in:
Published in: | Future microbiology 2020-03, Vol.15 (4), p.259-271 |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | Characterization of the ability of
DSM 15643 and DSM 20482 strains in the presence of Cu
and H
O
to reactive oxygen species generation.
Spectrophotometric ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) method was used.
Determination of: MIC for Cu
, H
O
and ABTS; survivability of
under atmospheric oxygen exposure; the level and rate constants of free radicals production by the bacteria.
in the presence of Cu
and H
O
is able to generate free radicals. Reactive oxygen species are produced mainly outside the bacterial cell, which suggests that outer membrane proteins may be involved in oxidative process. |
---|---|
ISSN: | 1746-0913 1746-0921 |
DOI: | 10.2217/fmb-2019-0010 |