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Protocol of proceedings with Fusobacterium nucleatum and optimization of ABTS method for detection of reactive oxygen species
Characterization of the ability of DSM 15643 and DSM 20482 strains in the presence of Cu and H O to reactive oxygen species generation. Spectrophotometric ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) method was used. Determination of: MIC for Cu , H O and ABTS; survivability of...
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| Published in: | Future microbiology 2020-03, Vol.15 (4), p.259-271 |
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| Main Authors: | , , , , , , , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c321t-48ff23ca53e399a069a6ab61b57227be5b6fe8d89d8b948b974e864c2abe55fb3 |
| container_end_page | 271 |
| container_issue | 4 |
| container_start_page | 259 |
| container_title | Future microbiology |
| container_volume | 15 |
| creator | Kędziora, Anna Lesiów, Monika Katarzyna Krupa, Katarzyna Korzeniowska-Kowal, Agnieszka Adamski, Ryszard Komarnicka, Urszula Katarzyna Stokowa-Sołtys, Kamila Bugla-Płoskońska, Gabriela Jeżowska-Bojczuk, Małgorzata |
| description | Characterization of the ability of
DSM 15643 and DSM 20482 strains in the presence of Cu
and H
O
to reactive oxygen species generation.
Spectrophotometric ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) method was used.
Determination of: MIC for Cu
, H
O
and ABTS; survivability of
under atmospheric oxygen exposure; the level and rate constants of free radicals production by the bacteria.
in the presence of Cu
and H
O
is able to generate free radicals. Reactive oxygen species are produced mainly outside the bacterial cell, which suggests that outer membrane proteins may be involved in oxidative process. |
| doi_str_mv | 10.2217/fmb-2019-0010 |
| format | article |
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DSM 15643 and DSM 20482 strains in the presence of Cu
and H
O
to reactive oxygen species generation.
Spectrophotometric ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) method was used.
Determination of: MIC for Cu
, H
O
and ABTS; survivability of
under atmospheric oxygen exposure; the level and rate constants of free radicals production by the bacteria.
in the presence of Cu
and H
O
is able to generate free radicals. Reactive oxygen species are produced mainly outside the bacterial cell, which suggests that outer membrane proteins may be involved in oxidative process.</description><identifier>ISSN: 1746-0913</identifier><identifier>EISSN: 1746-0921</identifier><identifier>DOI: 10.2217/fmb-2019-0010</identifier><identifier>PMID: 32271108</identifier><language>eng</language><publisher>England: Future Medicine Ltd</publisher><subject>Bacteria ; Binding sites ; Copper ; Experiments ; Free radicals ; Fusobacterium nucleatum ; Hydrogen peroxide ; Membrane proteins ; Minimum inhibitory concentration ; Outer membrane proteins ; Oxidation ; Oxidative stress ; Potassium ; Proteins ; Reactive oxygen species ; Spectrophotometry ; Tumorigenesis ; Tumors</subject><ispartof>Future microbiology, 2020-03, Vol.15 (4), p.259-271</ispartof><rights>Copyright Future Medicine Ltd Mar 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c321t-48ff23ca53e399a069a6ab61b57227be5b6fe8d89d8b948b974e864c2abe55fb3</citedby><cites>FETCH-LOGICAL-c321t-48ff23ca53e399a069a6ab61b57227be5b6fe8d89d8b948b974e864c2abe55fb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32271108$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kędziora, Anna</creatorcontrib><creatorcontrib>Lesiów, Monika Katarzyna</creatorcontrib><creatorcontrib>Krupa, Katarzyna</creatorcontrib><creatorcontrib>Korzeniowska-Kowal, Agnieszka</creatorcontrib><creatorcontrib>Adamski, Ryszard</creatorcontrib><creatorcontrib>Komarnicka, Urszula Katarzyna</creatorcontrib><creatorcontrib>Stokowa-Sołtys, Kamila</creatorcontrib><creatorcontrib>Bugla-Płoskońska, Gabriela</creatorcontrib><creatorcontrib>Jeżowska-Bojczuk, Małgorzata</creatorcontrib><title>Protocol of proceedings with Fusobacterium nucleatum and optimization of ABTS method for detection of reactive oxygen species</title><title>Future microbiology</title><addtitle>Future Microbiol</addtitle><description>Characterization of the ability of
DSM 15643 and DSM 20482 strains in the presence of Cu
and H
O
to reactive oxygen species generation.
Spectrophotometric ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) method was used.
Determination of: MIC for Cu
, H
O
and ABTS; survivability of
under atmospheric oxygen exposure; the level and rate constants of free radicals production by the bacteria.
in the presence of Cu
and H
O
is able to generate free radicals. Reactive oxygen species are produced mainly outside the bacterial cell, which suggests that outer membrane proteins may be involved in oxidative process.</description><subject>Bacteria</subject><subject>Binding sites</subject><subject>Copper</subject><subject>Experiments</subject><subject>Free radicals</subject><subject>Fusobacterium nucleatum</subject><subject>Hydrogen peroxide</subject><subject>Membrane proteins</subject><subject>Minimum inhibitory concentration</subject><subject>Outer membrane proteins</subject><subject>Oxidation</subject><subject>Oxidative stress</subject><subject>Potassium</subject><subject>Proteins</subject><subject>Reactive oxygen species</subject><subject>Spectrophotometry</subject><subject>Tumorigenesis</subject><subject>Tumors</subject><issn>1746-0913</issn><issn>1746-0921</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNpdkc1rFjEQh4Mo9kOPvZaAl15WM8luNntsi22FgoL1HJLspE3Z3bxNsmoL_u_NSz8OHoYMzDM_MjyEHAD7zDn0X_xsG85gaBgD9obsQt_Khg0c3r72IHbIXs63jHUKBnhPdgTnPQBTu-TfjxRLdHGi0dNNig5xDMt1pn9CuaFna47WuIIprDNdVjehKbUzy0jjpoQ5PJgS4rJdPj65-klnLDdxpD4mOmJB9zJMWFPCb6Tx7_01LjRv0AXMH8g7b6aMH5_fffLr7OvV6UVz-f382-nxZeMEh9K0ynsunOkEimEwTA5GGivBdn09xGJnpUc1qmFUdmhr9S0q2Tpu6qzzVuyTo6fceuHdirnoOWSH02QWjGvWXCjFGJcgK_rpP_Q2rmmpv9O8BZCyl11fqeaJcinmnNDrTQqzSfcamN560dWL3nrRWy-VP3xOXe2M4yv9IkI8AqpJioQ</recordid><startdate>202003</startdate><enddate>202003</enddate><creator>Kędziora, Anna</creator><creator>Lesiów, Monika Katarzyna</creator><creator>Krupa, Katarzyna</creator><creator>Korzeniowska-Kowal, Agnieszka</creator><creator>Adamski, Ryszard</creator><creator>Komarnicka, Urszula Katarzyna</creator><creator>Stokowa-Sołtys, Kamila</creator><creator>Bugla-Płoskońska, Gabriela</creator><creator>Jeżowska-Bojczuk, Małgorzata</creator><general>Future Medicine Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>EHMNL</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>202003</creationdate><title>Protocol of proceedings with Fusobacterium nucleatum and optimization of ABTS method for detection of reactive oxygen species</title><author>Kędziora, Anna ; Lesiów, Monika Katarzyna ; Krupa, Katarzyna ; Korzeniowska-Kowal, Agnieszka ; Adamski, Ryszard ; Komarnicka, Urszula Katarzyna ; Stokowa-Sołtys, Kamila ; Bugla-Płoskońska, Gabriela ; Jeżowska-Bojczuk, Małgorzata</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c321t-48ff23ca53e399a069a6ab61b57227be5b6fe8d89d8b948b974e864c2abe55fb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Bacteria</topic><topic>Binding sites</topic><topic>Copper</topic><topic>Experiments</topic><topic>Free radicals</topic><topic>Fusobacterium nucleatum</topic><topic>Hydrogen peroxide</topic><topic>Membrane proteins</topic><topic>Minimum inhibitory concentration</topic><topic>Outer membrane proteins</topic><topic>Oxidation</topic><topic>Oxidative stress</topic><topic>Potassium</topic><topic>Proteins</topic><topic>Reactive oxygen species</topic><topic>Spectrophotometry</topic><topic>Tumorigenesis</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kędziora, Anna</creatorcontrib><creatorcontrib>Lesiów, Monika Katarzyna</creatorcontrib><creatorcontrib>Krupa, Katarzyna</creatorcontrib><creatorcontrib>Korzeniowska-Kowal, Agnieszka</creatorcontrib><creatorcontrib>Adamski, Ryszard</creatorcontrib><creatorcontrib>Komarnicka, Urszula Katarzyna</creatorcontrib><creatorcontrib>Stokowa-Sołtys, Kamila</creatorcontrib><creatorcontrib>Bugla-Płoskońska, Gabriela</creatorcontrib><creatorcontrib>Jeżowska-Bojczuk, Małgorzata</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database (Proquest)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>UK & Ireland Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Future microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kędziora, Anna</au><au>Lesiów, Monika Katarzyna</au><au>Krupa, Katarzyna</au><au>Korzeniowska-Kowal, Agnieszka</au><au>Adamski, Ryszard</au><au>Komarnicka, Urszula Katarzyna</au><au>Stokowa-Sołtys, Kamila</au><au>Bugla-Płoskońska, Gabriela</au><au>Jeżowska-Bojczuk, Małgorzata</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protocol of proceedings with Fusobacterium nucleatum and optimization of ABTS method for detection of reactive oxygen species</atitle><jtitle>Future microbiology</jtitle><addtitle>Future Microbiol</addtitle><date>2020-03</date><risdate>2020</risdate><volume>15</volume><issue>4</issue><spage>259</spage><epage>271</epage><pages>259-271</pages><issn>1746-0913</issn><eissn>1746-0921</eissn><abstract>Characterization of the ability of
DSM 15643 and DSM 20482 strains in the presence of Cu
and H
O
to reactive oxygen species generation.
Spectrophotometric ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) method was used.
Determination of: MIC for Cu
, H
O
and ABTS; survivability of
under atmospheric oxygen exposure; the level and rate constants of free radicals production by the bacteria.
in the presence of Cu
and H
O
is able to generate free radicals. Reactive oxygen species are produced mainly outside the bacterial cell, which suggests that outer membrane proteins may be involved in oxidative process.</abstract><cop>England</cop><pub>Future Medicine Ltd</pub><pmid>32271108</pmid><doi>10.2217/fmb-2019-0010</doi><tpages>13</tpages></addata></record> |
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| identifier | ISSN: 1746-0913 |
| ispartof | Future microbiology, 2020-03, Vol.15 (4), p.259-271 |
| issn | 1746-0913 1746-0921 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2388002616 |
| source | PubMed Central |
| subjects | Bacteria Binding sites Copper Experiments Free radicals Fusobacterium nucleatum Hydrogen peroxide Membrane proteins Minimum inhibitory concentration Outer membrane proteins Oxidation Oxidative stress Potassium Proteins Reactive oxygen species Spectrophotometry Tumorigenesis Tumors |
| title | Protocol of proceedings with Fusobacterium nucleatum and optimization of ABTS method for detection of reactive oxygen species |
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