DNA topoisomerase 2 gene polymorphism in dermatophytes

Summary Background Dermatophytes are a group of keratinophilic fungi of medical importance. Despite a relatively long history of molecular taxonomic studies, there is still a need for information on genetic polymorphism in wider variety of genomic loci. Objectives Our goal was to study partial DNA t...

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Bibliographic Details
Published in:Mycoses 2020-07, Vol.63 (7), p.694-703
Main Authors: Shamsizadeh, Forough, Pchelin, Ivan M., Makimura, Koichi, Alshahni, Mohamed Mahdi, Satoh, Kazuo, Katiraee, Farzad, Ahmadi, Bahram, Rezaei‐Matehhkolaei, Ali
Format: Article
Language:English
Subjects:
Arthrodermataceae - classification
Arthrodermataceae - enzymology
Arthrodermataceae - genetics
Deoxyribonucleic acid
dermatophytes
DNA
DNA sequencing
DNA topoisomerase
DNA topoisomerase 2
DNA Topoisomerases, Type II - genetics
DNA, Fungal - genetics
DNA, Ribosomal Spacer - genetics
Gene polymorphism
Iran
Keratinophilic fungi
Medical importance
Nannizzia
Phylogeny
Polymorphism
Polymorphism, Genetic
Sequence Analysis, DNA
Species
species boundaries
Taxonomy
Trichophyton
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Summary:Summary Background Dermatophytes are a group of keratinophilic fungi of medical importance. Despite a relatively long history of molecular taxonomic studies, there is still a need for information on genetic polymorphism in wider variety of genomic loci. Objectives Our goal was to study partial DNA topoisomerase 2 gene (TOP2) polymorphism in dermatophytes. Methods We performed DNA sequencing of TOP2 in 26 dermatophyte species along with ribosomal internal transcribed spacer (ITS) sequencing. Results The number of polymorphic sites in TOP2 data set was similar to that one in ITS data set. Nannizzia species formed paraphyletic group in TOP2 tree. Trichophyton simii was paraphyletic in concatenated TOP2‐ITS tree, one of its two clades contained solely Iranian isolates. Conclusions Our results revealed several unresolved problems in the taxonomy of dermatophytes, including probable polyphyly of the genus Nannizzia and the species T simii.
ISSN:0933-7407
1439-0507
DOI:10.1111/myc.13086