Analysis of chemical constituents in the extract and rat serum from the chloroform extract of Oxytropis falcata Bunge by HPLC-MS

Analysis of the constituents of the chloroform extract of Oxytropis falcata Bunge (CEOF), a traditional Tibetan medicine, in rat's serum after oral administration, has been performed by HPLC-MS. We have identified 10 compounds in CEOF and 11 bioactive ingredients from rat's serum after giv...

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Bibliographic Details
Published in:Pakistan journal of pharmaceutical sciences 2020-03, Vol.33 (2), p.669-674
Main Authors: Zhang, Dejun, Yuan, Wangtao, Zhang, Benyin, Hong, Eun Kyung, Shi, Ping, Yang, Zhanting, Zhang, Yuhao, Wang, Honglun
Format: Article
Language:English
Subjects:
Animals
Chloroform - analysis
Chloroform - chemistry
Chromatography, High Pressure Liquid - methods
Female
Male
Microsomes, Liver - chemistry
Microsomes, Liver - metabolism
Oxytropis
Plant Extracts - analysis
Plant Extracts - chemistry
Rats
Rats, Sprague-Dawley
Tandem Mass Spectrometry - methods
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Summary:Analysis of the constituents of the chloroform extract of Oxytropis falcata Bunge (CEOF), a traditional Tibetan medicine, in rat's serum after oral administration, has been performed by HPLC-MS. We have identified 10 compounds in CEOF and 11 bioactive ingredients from rat's serum after given CEOF. Six bioactive ingredients from rat's serum are matched with original form of the compounds of CEOF. Other five bioactive ingredients were seemed to be respectively metabolites. HPLC-MS is rapid, sensitive method and suitable for identification of bioactive components absorbed into blood of CEOF providing information for further research of pharmacological mechanism.
ISSN:1011-601X
DOI:10.36721/PJPS.2020.33.2.REG.669-674.1