Mitochondria determine the sequential propagation of the calcium macrodomains revealed by the super-resolution calcium lantern imaging

Despite the wide application of super-resolution (SR) microscopy in biological studies of cells, the technology is rarely used to monitor functional changes in live cells. By combining fast spinning disc-confocal structured illumination microscopy (SD-SIM) with loading of cytosolic fluorescent Ca 2+...

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Bibliographic Details
Published in:Science China. Life sciences 2020-10, Vol.63 (10), p.1543-1551
Main Authors: Zhang, Yulin, Wang, Jianyong, Xing, Shijia, Li, Liuju, Zhao, Shiqun, Zhu, Wenzhen, Liang, Kuo, Liu, Yanmei, Chen, Liangyi
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Calcium (reticular)
Calcium imaging
Calcium influx
Calcium signalling
Cytology
Endoplasmic reticulum
Life Sciences
Microscopy
Mitochondria
Research Paper
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Summary:Despite the wide application of super-resolution (SR) microscopy in biological studies of cells, the technology is rarely used to monitor functional changes in live cells. By combining fast spinning disc-confocal structured illumination microscopy (SD-SIM) with loading of cytosolic fluorescent Ca 2+ indicators, we have developed an SR method for visualization of regional Ca 2+ dynamics and related cellular organelle morphology and dynamics, termed SR calcium lantern imaging. In COS-7 cells stimulated with ATP, we have identified various calcium macrodomains characterized by different types of Ca 2 + release from endoplasmic reticulum (ER) stores. Finally, we demonstrated various roles of mitochondria in mediating calcium signals from different sources; while mitochondria can globally potentiate the Ca 2 + entry associated with store release, mitochondria also locally control Ca 2+ release from the neighboring ER stores and assist in their refilling processes.
ISSN:1674-7305
1869-1889
DOI:10.1007/s11427-019-1659-4