Rapid identification of chemical compositions in callicarpa kwangtungensis Chun by ultra‐high‐performance liquid chromatography with Q Exactive hybrid quadrupole orbitrap high‐resolution accurate mass spectrometry

Callicarpa kwangtungensis Chun is a traditional Chinese medicine that has various therapeutic effects. Despite its wide use in Chinese medicine, the study is still quite limited, especially its chemical compositions. In this research, an ultra‐high‐pressure liquid chromatography coupled with Q Exact...

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Bibliographic Details
Published in:Journal of separation science 2020-06, Vol.43 (12), p.2487-2494
Main Authors: Yang, Lanping, Zhai, Yangyang, Chen, Xingying, Zhang, Zhenzhen, Gong, Xueyun, Zhou, Dejun, Kong, Jichuan, Zhang, Wenkui, Zhang, Qingling, Niu, Chao, Wang, Zhenhui, Sun, Zhi
Format: Article
Language:English
Subjects:
Callicarpa - chemistry
Callicarpa kwangtungensis Chun
Chemical composition
chemical compositions
Chinese medicine
Chromatography
Chromatography, High Pressure Liquid
Drugs, Chinese Herbal - analysis
Flavonoids
Glycosides
Liquid chromatography
Mass Spectrometry
Medicine, Chinese Traditional
Molecular Structure
Organic acids
Phenols
Quadrupoles
Quality control
Scientific imaging
Spectroscopy
tandem mass spectrometry
Terpenes
Traditional Chinese medicine
ultra‐high‐pressure liquid chromatography
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Summary:Callicarpa kwangtungensis Chun is a traditional Chinese medicine that has various therapeutic effects. Despite its wide use in Chinese medicine, the study is still quite limited, especially its chemical compositions. In this research, an ultra‐high‐pressure liquid chromatography coupled with Q Exactive hybrid quadrupole‐orbitrap high‐resolution accurate mass spectrometry tandem mass spectrometry method was utilized to analyze its chemical compositions for the first time. As a result, a total of 124 compounds, including 20 phenylethanoid glycosides, 31 flavonoids, 36 organic acids, 26 terpenoids and 11 phenols, were identified or tentatively characterized in 30 min. Among them, 49 compounds, including 5 phenylethanoid glycosides, 12 flavonoids, 16 organic acids, 12 terpenoids, and 4 phenols, were identified in Callicarpa kwangtungensis Chun for the first time. Besides, the fragmentation pathways were also discussed. This research established a rapid and reliable method to analyze the chemical compositions of complicated herb without the process of isolation, and provide abundant information on the chemical material basis for further bioactivity and quality control studies.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.202000165