Extinction of cue‐evoked food‐seeking recruits a GABAergic interneuron ensemble in the dorsal medial prefrontal cortex of mice

• Published in: The European journal of neuroscience 2020-10, Vol.52 (7), p.3723-3737
• Main Authors: Brebner, Leonie S., Ziminski, Joseph J., Margetts‐Smith, Gabriella, Sieburg, Meike C., Hall, Catherine N., Heintz, Tristan G., Lagnado, Leon, Hirrlinger, Johannes, Crombag, Hans S., Koya, Eisuke
• Format: Article
• Language: English
• Subjects: Adaptation; Animals; Appetitive conditioning; Conditioning, Operant; Cues; Excitability; Extinction behavior; Extinction, Psychological; Food; Food availability; Green fluorescent protein; in vivo 2‐Photon imaging; Interneurons; Learning; Male; Mice; Neuroimaging; neuronal ensemble; neuronal excitability; Neurons; Nutrient sources; Prefrontal Cortex; Pyramidal cells; Reinforcement; Reward; γ-Aminobutyric acid
• ISSN: 0953-816X; 1460-9568
• DOI: 10.1111/ejn.14754

Animals must quickly adapt food‐seeking strategies to locate nutrient sources in dynamically changing environments. Learned associations between food and environmental cues that predict its availability promote food‐seeking behaviors. However, when such cues cease to predict food availability, animals undergo “extinction” learning, resulting in the inhibition of food‐seeking responses. Repeatedly activated sets of neurons, or “neuronal ensembles,” in the dorsal medial prefrontal cortex (dmPFC) are recruited following appetitive conditioning and undergo physiological adaptations thought to encode cue‐reward associations. However, little is known about how the recruitment and intrinsic excitability of such dmPFC ensembles are modulated by extinction learning. Here, we used in vivo 2‐Photon imaging in male Fos‐GFP mice that express green fluorescent protein (GFP) in recently behaviorally activated neurons to determine the recruitment of activated pyramidal and GABAergic interneuron dmPFC ensembles during extinction. During extinction, we revealed a persistent activation of a subset of interneurons which emerged from a wider population of interneurons activated during the initial extinction session. This activation pattern was not observed in pyramidal cells, and extinction learning did not modulate the excitability properties of activated pyramidal cells. Moreover, extinction learning reduced the likelihood of reactivation of pyramidal cells activated during the initial extinction session. Our findings illuminate novel neuronal activation patterns in the dmPFC underlying extinction of food‐seeking, and in particular, highlight an important role for interneuron ensembles in this inhibitory form of learning. Animals undergo “extinction” learning when cues that predict food availability no longer do so, which results in the inhibition of food‐seeking responses. Using in vivo imaging in Fos‐GFP mice, we revealed a persistent activation of a subset of interneurons or “interneuron ensemble” which emerged from a wider population of interneurons activated during the initial extinction session. This activation pattern was not observed in pyramidal cells. We did not observe excitability alterations in activated pyramidal cells during extinction learning. Our findings highlight an important role for interneuron ensembles in extinction learning.