Quantification of Trichoderma afroharzianum, Trichoderma harzianum and Trichoderma gamsii inoculants in soil, the wheat rhizosphere and in planta suppression of the crown rot pathogen Fusarium pseudograminearum

Aims Develop quantitative assays (qPCR) to determine the detection threshold limits, colonization and persistence of Trichoderma gamsii, Trichoderma afroharzianum and T. harzianum inoculants in cropping soils, the wheat rhizosphere and their in planta suppressive efficacy against the crown rot patho...

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Bibliographic Details
Published in:Journal of applied microbiology 2020-10, Vol.129 (4), p.971-990
Main Authors: Stummer, B.E., Zhang, Q., Zhang, X., Warren, R.A., Harvey, P.R.
Format: Article
Language:English
Subjects:
Assaying
Bioassays
biocontrol
Biological Control Agents
Biomass
Colonization
Conidia
Crown rot
Deoxyribonucleic acid
Diagnostic systems
DNA
DNA, Fungal - genetics
Edible Grain - growth & development
Edible Grain - microbiology
Equivalence
Fungi
Fusarium
Fusarium - physiology
Fusarium pseudograminearum
Gene sequencing
Genomes
Genotypes
markers
Nucleotide sequence
Pathogens
Plant biomass
plant diseases
Plant Diseases - microbiology
Plant Diseases - prevention & control
Plant Roots - microbiology
Plant tissues
Rhizosphere
soil
Soil Microbiology
Soils
Spacer region
Threshold limits
Trichoderma
Trichoderma - classification
Trichoderma - genetics
Trichoderma - isolation & purification
Trichoderma - physiology
Triticum - growth & development
Triticum - microbiology
Wheat
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Summary:Aims Develop quantitative assays (qPCR) to determine the detection threshold limits, colonization and persistence of Trichoderma gamsii, Trichoderma afroharzianum and T. harzianum inoculants in cropping soils, the wheat rhizosphere and their in planta suppressive efficacy against the crown rot pathogen Fusarium pseudograminearum. Methods and Results Trichoderma qPCR primers were designed from the internal transcribed spacer region of 5.8S rDNA and from sequences of DNA fragments diagnostic for each inoculant genotype. The minimum detection thresholds of qPCR assays varied between 1 × 103 (log 3) and 8 × 104 (log 4·9) conidia (genome) equivalents per gram of soil for multi‐ and single‐copy target sequences, respectively and were independent of soil type. There was a strong correlation (r > 0·974) between culture‐dependent and culture‐independent (qPCR) quantification methods. In wheat bioassays, Trichoderma inoculants colonized rhizosphere soils and wheat roots at 56–112 days postemergence to a depth of 20 cm but were more abundant (P 
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.14670