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Stepwise Control of Crosslinking in a One‐Pot System for Bioprinting of Low‐Density Bioinks
Extrusion‐based 3D bioprinting is hampered by the inability to print materials of low‐viscosity. In this study, a single initiating system based on ruthenium (Ru) and sodium persulfate (SPS) is utilized for a sequential dual‐step crosslinking approach: 1) primary (partial) crosslinking in absence of...
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Published in: | Advanced healthcare materials 2020-08, Vol.9 (15), p.e1901544-n/a |
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creator | Soliman, Bram G. Lindberg, Gabriella C. J. Jungst, Tomasz Hooper, Gary J. Groll, Jürgen Woodfield, Tim B. F. Lim, Khoon S. |
description | Extrusion‐based 3D bioprinting is hampered by the inability to print materials of low‐viscosity. In this study, a single initiating system based on ruthenium (Ru) and sodium persulfate (SPS) is utilized for a sequential dual‐step crosslinking approach: 1) primary (partial) crosslinking in absence of light to alter the bioink's rheological profile for print fidelity, and 2) subsequent secondary post‐printing crosslinking for shape maintenance. Allyl‐functionalized gelatin (Gel‐AGE) is used as a bioink, allowing thiol‐ene click reaction between allyl moieties and thiolated crosslinkers. A systematic investigation of primary crosslinking reveals that a thiol‐persulfate redox reaction facilitates thiol‐ene crosslinking, mediating an increase in bioink viscosity that is controllable by tailoring the Ru/SPS, crosslinker, and/or Gel‐AGE concentrations. Thereafter, subsequent photoinitiated secondary crosslinking then facilitates maximum conversion of thiol‐ene bonds between AGE and thiol groups. The dual‐step crosslinking method is applicable to a wide biofabrication window (3–10 wt% Gel‐AGE) and is demonstrated to allow printing of low‐density (3 wt%) Gel‐AGE, normally exhibiting low viscosity (4 mPa s), with high shape fidelity and high cell viability (>80%) over 7 days of culture. The presented approach can therefore be used as a one‐pot system for printing low‐viscous bioinks without the need for multiple initiating systems, viscosity enhancers, or complex chemical modifications.
Low density allyl‐functionalized gelatin bioinks are printable by step‐wise control of the degree of crosslinking to modify the rheological profile. The low‐density bioinks are irradiated with light to stabilize the construct, and are able to promote cell survival and function. |
doi_str_mv | 10.1002/adhm.201901544 |
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Low density allyl‐functionalized gelatin bioinks are printable by step‐wise control of the degree of crosslinking to modify the rheological profile. The low‐density bioinks are irradiated with light to stabilize the construct, and are able to promote cell survival and function.</description><identifier>ISSN: 2192-2640</identifier><identifier>EISSN: 2192-2659</identifier><identifier>DOI: 10.1002/adhm.201901544</identifier><identifier>PMID: 32323473</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Accuracy ; Age ; Bioengineering ; biofabrication ; bioinks ; Bioprinting ; Cell culture ; Cell viability ; Chemical reactions ; Crosslinking ; Density ; Extrusion ; Gelatin ; Ink ; low‐density bioinks ; Printing ; Printing, Three-Dimensional ; Rheological properties ; Rheology ; Ruthenium ; Sodium persulfate ; Stability ; Three dimensional printing ; Viscosity</subject><ispartof>Advanced healthcare materials, 2020-08, Vol.9 (15), p.e1901544-n/a</ispartof><rights>2020 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>2020 Wiley‐VCH GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4214-9c3d2502aae72064deba1bc3b8f56b2e3f3f87b61b422c35a7114353c3c705583</citedby><cites>FETCH-LOGICAL-c4214-9c3d2502aae72064deba1bc3b8f56b2e3f3f87b61b422c35a7114353c3c705583</cites><orcidid>0000-0002-2486-196X ; 0000-0003-3167-8466 ; 0000-0002-5428-7575 ; 0000-0003-1575-2128</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32323473$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Soliman, Bram G.</creatorcontrib><creatorcontrib>Lindberg, Gabriella C. J.</creatorcontrib><creatorcontrib>Jungst, Tomasz</creatorcontrib><creatorcontrib>Hooper, Gary J.</creatorcontrib><creatorcontrib>Groll, Jürgen</creatorcontrib><creatorcontrib>Woodfield, Tim B. F.</creatorcontrib><creatorcontrib>Lim, Khoon S.</creatorcontrib><title>Stepwise Control of Crosslinking in a One‐Pot System for Bioprinting of Low‐Density Bioinks</title><title>Advanced healthcare materials</title><addtitle>Adv Healthc Mater</addtitle><description>Extrusion‐based 3D bioprinting is hampered by the inability to print materials of low‐viscosity. In this study, a single initiating system based on ruthenium (Ru) and sodium persulfate (SPS) is utilized for a sequential dual‐step crosslinking approach: 1) primary (partial) crosslinking in absence of light to alter the bioink's rheological profile for print fidelity, and 2) subsequent secondary post‐printing crosslinking for shape maintenance. Allyl‐functionalized gelatin (Gel‐AGE) is used as a bioink, allowing thiol‐ene click reaction between allyl moieties and thiolated crosslinkers. A systematic investigation of primary crosslinking reveals that a thiol‐persulfate redox reaction facilitates thiol‐ene crosslinking, mediating an increase in bioink viscosity that is controllable by tailoring the Ru/SPS, crosslinker, and/or Gel‐AGE concentrations. Thereafter, subsequent photoinitiated secondary crosslinking then facilitates maximum conversion of thiol‐ene bonds between AGE and thiol groups. The dual‐step crosslinking method is applicable to a wide biofabrication window (3–10 wt% Gel‐AGE) and is demonstrated to allow printing of low‐density (3 wt%) Gel‐AGE, normally exhibiting low viscosity (4 mPa s), with high shape fidelity and high cell viability (>80%) over 7 days of culture. The presented approach can therefore be used as a one‐pot system for printing low‐viscous bioinks without the need for multiple initiating systems, viscosity enhancers, or complex chemical modifications.
Low density allyl‐functionalized gelatin bioinks are printable by step‐wise control of the degree of crosslinking to modify the rheological profile. The low‐density bioinks are irradiated with light to stabilize the construct, and are able to promote cell survival and function.</description><subject>Accuracy</subject><subject>Age</subject><subject>Bioengineering</subject><subject>biofabrication</subject><subject>bioinks</subject><subject>Bioprinting</subject><subject>Cell culture</subject><subject>Cell viability</subject><subject>Chemical reactions</subject><subject>Crosslinking</subject><subject>Density</subject><subject>Extrusion</subject><subject>Gelatin</subject><subject>Ink</subject><subject>low‐density bioinks</subject><subject>Printing</subject><subject>Printing, Three-Dimensional</subject><subject>Rheological properties</subject><subject>Rheology</subject><subject>Ruthenium</subject><subject>Sodium persulfate</subject><subject>Stability</subject><subject>Three dimensional printing</subject><subject>Viscosity</subject><issn>2192-2640</issn><issn>2192-2659</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqFkMFOAjEQhhujEYJcPZomXryA7bTdZY8IKiYYTdBz0126WtxtcbuEcPMRfEafxG5ATLzYObTJfPOn8yF0SkmfEgKXav5a9oHQhFDB-QFqA02gB5FIDvdvTlqo6_2ChBMJGg3oMWoxCMVj1kZyVuvl2niNR87WlSuwy_Goct4Xxr4Z-4KNxQo_WP318fnoajzb-FqXOHcVvjJuWRlbN1SYmrp1YMbaelNvmmYI8CfoKFeF193d3UHPN9dPo0lv-nB7NxpOexkHyntJxuYgCCilYyARn-tU0TRj6SAXUQqa5SwfxGlEUw6QMaFiSjkTLGNZTIQYsA662OYuK_e-0r6WpfGZLgpltVt5CSzhIBLgLKDnf9CFW1U2_E6GNmFAgEaB6m-prJFR6VyGXUtVbSQlsrEvG_tybz8MnO1iV2mp53v8x3UAki2wNoXe_BMnh-PJ_W_4Nz5ikT4</recordid><startdate>20200801</startdate><enddate>20200801</enddate><creator>Soliman, Bram G.</creator><creator>Lindberg, Gabriella C. 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J. ; Jungst, Tomasz ; Hooper, Gary J. ; Groll, Jürgen ; Woodfield, Tim B. 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J.</au><au>Jungst, Tomasz</au><au>Hooper, Gary J.</au><au>Groll, Jürgen</au><au>Woodfield, Tim B. F.</au><au>Lim, Khoon S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stepwise Control of Crosslinking in a One‐Pot System for Bioprinting of Low‐Density Bioinks</atitle><jtitle>Advanced healthcare materials</jtitle><addtitle>Adv Healthc Mater</addtitle><date>2020-08-01</date><risdate>2020</risdate><volume>9</volume><issue>15</issue><spage>e1901544</spage><epage>n/a</epage><pages>e1901544-n/a</pages><issn>2192-2640</issn><eissn>2192-2659</eissn><abstract>Extrusion‐based 3D bioprinting is hampered by the inability to print materials of low‐viscosity. In this study, a single initiating system based on ruthenium (Ru) and sodium persulfate (SPS) is utilized for a sequential dual‐step crosslinking approach: 1) primary (partial) crosslinking in absence of light to alter the bioink's rheological profile for print fidelity, and 2) subsequent secondary post‐printing crosslinking for shape maintenance. Allyl‐functionalized gelatin (Gel‐AGE) is used as a bioink, allowing thiol‐ene click reaction between allyl moieties and thiolated crosslinkers. A systematic investigation of primary crosslinking reveals that a thiol‐persulfate redox reaction facilitates thiol‐ene crosslinking, mediating an increase in bioink viscosity that is controllable by tailoring the Ru/SPS, crosslinker, and/or Gel‐AGE concentrations. Thereafter, subsequent photoinitiated secondary crosslinking then facilitates maximum conversion of thiol‐ene bonds between AGE and thiol groups. The dual‐step crosslinking method is applicable to a wide biofabrication window (3–10 wt% Gel‐AGE) and is demonstrated to allow printing of low‐density (3 wt%) Gel‐AGE, normally exhibiting low viscosity (4 mPa s), with high shape fidelity and high cell viability (>80%) over 7 days of culture. The presented approach can therefore be used as a one‐pot system for printing low‐viscous bioinks without the need for multiple initiating systems, viscosity enhancers, or complex chemical modifications.
Low density allyl‐functionalized gelatin bioinks are printable by step‐wise control of the degree of crosslinking to modify the rheological profile. The low‐density bioinks are irradiated with light to stabilize the construct, and are able to promote cell survival and function.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>32323473</pmid><doi>10.1002/adhm.201901544</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-2486-196X</orcidid><orcidid>https://orcid.org/0000-0003-3167-8466</orcidid><orcidid>https://orcid.org/0000-0002-5428-7575</orcidid><orcidid>https://orcid.org/0000-0003-1575-2128</orcidid></addata></record> |
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subjects | Accuracy Age Bioengineering biofabrication bioinks Bioprinting Cell culture Cell viability Chemical reactions Crosslinking Density Extrusion Gelatin Ink low‐density bioinks Printing Printing, Three-Dimensional Rheological properties Rheology Ruthenium Sodium persulfate Stability Three dimensional printing Viscosity |
title | Stepwise Control of Crosslinking in a One‐Pot System for Bioprinting of Low‐Density Bioinks |
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