Establishing practical strategies to run high loading corn stover anaerobic digestion: Methane production performance and microbial responses

•TS was increased gradually until CS AD system was acidized.•Methane production rate was the highest in 13% TS, 15% TS led to acidification.•Adding 30% CM could rescue AD system back to a stable operation.•Acidification of CS was highly correlating with Clostridium and Caproiciproducens.•High abunda...

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Published in:Bioresource technology 2020-08, Vol.310, p.123364-123364, Article 123364
Main Authors: Wang, Haipeng, Li, Jiawei, Zhao, Yiquan, Xu, Congfeng, Zhang, Keqiang, Li, Jiajia, Yan, Lei, Gu, Ji-Dong, Wei, Dan, Wang, Weidong
Format: Article
Language:English
Subjects:
Acidification
Anaerobic digestion
Anaerobiosis
Animals
Archaea
bacterial communities
Biofuels
Bioreactors
Cattle
cattle manure
Clostridium
Corn stover
gas production (biological)
genes
Manure
Methane
methane production
Microbial diversity
Organic loading
RNA, Ribosomal, 16S
Zea mays
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Summary:•TS was increased gradually until CS AD system was acidized.•Methane production rate was the highest in 13% TS, 15% TS led to acidification.•Adding 30% CM could rescue AD system back to a stable operation.•Acidification of CS was highly correlating with Clostridium and Caproiciproducens.•High abundance (31.07%) of Bathyarchaeota was found in AD system. It is significant to understand corn stover (CS) in anaerobic digestion (AD) under high organic loadings. A semi-continuous mesophilic (37 ± 1 °C) CS AD was conducted in this study with increasing loadings. The initial total solids (TS) gradually increased with 1% gradient at every 10 days from 8% to 15% until the system was acidified. Adding different ratios of cattle manure (CM) (20%, 30% and 40% (v/v)) to rescue this system back to a stable operation was adopted. The diversity of bacteria and archaea was analyzed by 16S rRNA gene sequencing technology. The results showed that when loading TS content was increased to 15%, AD system was acidized with pH value of 5.13. 30% of CM was the optimal ratio to recover biogas production. High abundance (31.07%) of Bathyarchaeota was first found in AD system. Acidification of high loading CS AD can be highly correlating with bacterial community, specially Clostridium and Caproiciproducens.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2020.123364