Molecularly imprinted cryogel cartridges for the selective recognition of tyrosine

Molecularly imprinted polymers are used for creating a specific cavity and selective recognition sites for the structure of a target molecule in a polymeric structure. In this study, specific molecularly imprinted cryogel cartridges were synthesized using two distinct functional monomers to compare...

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Bibliographic Details
Published in:Biotechnology progress 2020-09, Vol.36 (5), p.e3006-n/a
Main Authors: Bakhshpour, Monireh, Göktürk, Ilgım, Bereli, Nilay, Denizli, Adil
Format: Article
Language:English
Subjects:
Adsorption
Bulk polymerization
Cartridges
cryogel cartridges
Flow rates
Flow velocity
hydrophobic interaction
Hydrophobicity
Imprinted polymers
metal‐chelate coordination
molecular imprinting
Molecular structure
Monomers
Phenylalanine
Polymers
Recognition
Selectivity
Tryptophan
Tyrosine
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Summary:Molecularly imprinted polymers are used for creating a specific cavity and selective recognition sites for the structure of a target molecule in a polymeric structure. In this study, specific molecularly imprinted cryogel cartridges were synthesized using two distinct functional monomers to compare imprinting efficiency for the selective recognition of Tyrosine (Tyr). Tyr‐imprinted cryogel cartridge (MIP1) was prepared using metal‐chelate coordination for the imprinting process by free‐radical bulk polymerization under frozen conditions, and Tyr‐imprinted cryogel cartridge (MIP2) was prepared in the same way using hydrophobic effects for imprinting. After the characterization of the cryogel cartridges was carried out, the optimum adsorption conditions of both were determined according to the different parameters such as flow rate (0.5–2.5 ml/min), pH of the medium (4.0–8.0), initial Tyr concentration (0.1–3.0 mg/ml), and temperature (4–45°C). Selectivity experiments of Tyr‐imprinted and non‐imprinted cryogel cartridges were carried out by using phenylalanine, tryptophan, and cysteine. Besides, the eluted Tyr from MIP1 and MIP2 cryogel cartridge were applied to FPLC system. Also, the reusability experiments of Tyr‐imprinted cryogel cartridges was observed no significant decrease in the adsorption capacity.
ISSN:8756-7938
1520-6033
DOI:10.1002/btpr.3006