Monitoring and Modeling of Living Cell Responses in the Attachment Process and Reaction to the Antitumor Reagent Cisplatin Studied by a Quartz Crystal Microbalance Combined with a Microscope

The attachment process and response to an antitumor reagent for cultured cells were monitored with a quartz crystal microbalance (QCM) combined with a microscope. To fit the experimentally obtained curves of the resonant frequency, model equations of resonant frequency curves were built, and paramet...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2020-06, Vol.92 (11), p.7907-7914
Main Authors: Muramatsu, Hiroshi, Ito, Sae, Alsaleem, Abdullah Hussain A
Format: Article
Language:English
Subjects:
Antineoplastic Agents - chemistry
Antineoplastic Agents - pharmacology
Antitumor activity
Attachment
Cell adhesion
Cell Adhesion - drug effects
Cell Communication - drug effects
Cell number
Chemistry
Chemotherapy
Cisplatin
Cisplatin - chemistry
Cisplatin - pharmacology
Distribution functions
Hep G2 Cells
Humans
Interaction models
Microbalances
Microscopy
Models, Molecular
Normal distribution
Particle Size
Quartz
Quartz crystal microbalance
Quartz Crystal Microbalance Techniques
Quartz crystals
Reagents
Resonant frequencies
Surface Properties
Tumor Cells, Cultured
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Summary:The attachment process and response to an antitumor reagent for cultured cells were monitored with a quartz crystal microbalance (QCM) combined with a microscope. To fit the experimentally obtained curves of the resonant frequency, model equations of resonant frequency curves were built, and parameters of time constants and scale coefficients were determined. For the cell attachment process, a first-order lag response curve well fit the experimental curves. For the response to cisplatin, two response steps were observed in both QCM data and microscopic images, where the cells loosened in the first step and shrank in the second step. Resonant frequency responses for both processes were well fit by two logarithmic normal distribution functions. In addition, the dependence of the resonant frequency change on the cell number was also studied, and a cell-cell interaction model for attached cells was proposed to explain the saturation of the resonant frequency change in high density cell seeding.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.0c01274