Ulvan lyase assisted structural characterization of ulvan from Ulva pertusa and its antiviral activity against vesicular stomatitis virus

• Published in: International journal of biological macromolecules 2020-08, Vol.157, p.75-82
• Main Authors: Chi, Yongzhou, Zhang, Meifang, Wang, Xin, Fu, Xianjun, Guan, Huashi, Wang, Peng
• Format: Article
• Language: English
• Subjects: Antiviral activity; Antiviral Agents - chemistry; Antiviral Agents - metabolism; Antiviral Agents - pharmacology; Carbohydrate Sequence; Molecular Weight; Polysaccharide-Lyases - metabolism; Polysaccharides - chemistry; Polysaccharides - metabolism; Polysaccharides - pharmacology; Structure; Sulfated polysaccharide; Ulva - enzymology; Ulva pertusa; Ulvan lyase; Vesiculovirus - drug effects; Vesiculovirus - physiology; Virus Replication - drug effects
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2020.04.187

Marine green algae are valuable sources of diverse health-promoting bioactive components. Ulvan is suitable for biological applications due to its unique structure and numerous bioactivities. Here, the complex structure of ulvan from Ulva pertusa was analyzed using specific ulvan lyase degradation, MS, and NMR detection. Its structure mainly consists of →4)-β-d-GlcA-(1 → 4)-α-l-Rha3S-(1 → and →4)-β-d-Xyl-(1 → 4)-α-l-Rha3S-(1 → repeating units. Small amounts of →4)-α-l-IdoA-(1 → 4)-α-l-Rha3S-(1 → unit also exist. In addition, a minor number of branches, a single GlcA, and a long branch containing GlcA-Glc were linked to Rha3S. The antiviral activity of the ulvan and its degraded fragments were further investigated. Ulvan (1068.2 kDa) and ulvan-F1 (38.5 kDa) with relatively high molecular weight showed potency of inhibiting the infection and replication of vesicular stomatitis virus (VSV) at 100 μg/mL, the inhibition rate of VSV replication was 40.75% and 40.13%, respectively. These results indicated that ulvan has potential as a functional agent. •An ulvan lyase was used for the structure analysis of ulvan-F0 from Ulva pertusa.•Ulvan-F0 consists of three repeating disaccharide units and two side branches.•Molecular weight significantly affects the antiviral activity of ulvan.•Ulvan-F0 and ulvan-F1 (38.5 kDa) could inhibit the infection and replication of VSV.