Three-dimensional interferometric scattering microscopy via remote focusing technique

Interferometric scattering (iSCAT) microscopy enables us to track nm-sized objects with high spatial and temporal resolutions and permits label-free imaging of biomolecules. Its superb sensitivity, however, comes at a cost by several downsides, such as slow three-dimensional imaging and limited vert...

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Bibliographic Details
Published in:Optics letters 2020-05, Vol.45 (9), p.2628-2631
Main Authors: Lee, Il-Buem, Moon, Hyeon-Min, Park, Jin-Sung, Zambochova, Katerina, Hong, Seok-Cheol, Cho, Minhaeng
Format: Article
Language:English
Subjects:
Beads
Biomolecules
Imaging
Imaging, Three-Dimensional - instrumentation
Interferometry
Microscopy
Microscopy - instrumentation
Optical Phenomena
Scattering
Tracking
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Summary:Interferometric scattering (iSCAT) microscopy enables us to track nm-sized objects with high spatial and temporal resolutions and permits label-free imaging of biomolecules. Its superb sensitivity, however, comes at a cost by several downsides, such as slow three-dimensional imaging and limited vertical tracking. Here, we propose a new method, Remote Focusing-iSCAT (RF-iSCAT) microscopy, to visualize a volume specimen by imaging sections at different depths without translation of either the objective lens or sample stage. We demonstrate the principle of RF-iSCAT by determining the -position of submicrometer beads by translating the reference mirror instead. RF-iSCAT features an unprecedentedly long range of vertical tracking and permits fast but vibration-free vertical scanning. We anticipate that RF-iSCAT would enhance the utility of iSCAT for dynamics study.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.386172