Electrical stimulation of the carotid sinus lowers arterial pressure and improves heart rate variability in L-NAME hypertensive conscious rats

• Published in: Hypertension research 2020-10, Vol.43 (10), p.1057-1067
• Main Authors: Domingos-Souza, Gean, Santos-Almeida, Fernanda Machado, Meschiari, César Arruda, Ferreira, Nathanne S, Pereira, Camila A, Martinez, Diana, Dias, Daniel Penteado Martins, Silva, Luiz Eduardo Virgílio, Castania, Jaci Airton, Tostes, Rita C, Fazan, Jr, Rubens
• Format: Article
• Language: English
• Subjects: Animals; Arterial Pressure; Baroreflex; Disease Models, Animal; Electric Stimulation Therapy - methods; Electrodes; Heart Rate; Hypertension; Hypertension - enzymology; Hypertension - physiopathology; Hypertension - therapy; In Vitro Techniques; Male; Mesenteric Arteries - enzymology; NG-Nitroarginine Methyl Ester; Nitric Oxide Synthase Type III - metabolism; Rats, Wistar; Rodents
• ISSN: 0916-9636; 1348-4214
• DOI: 10.1038/s41440-020-0448-7

We evaluated the effects of long-term (48 h) electrical stimulation of the carotid sinus (CS) in hypertensive rats. L-NAME-treated (10 days) Wistar rats were implanted with a catheter in the femoral artery and a miniaturized electrical stimulator attached to electrodes positioned around the left CS, encompassing the CS nerve. One day after implantation, arterial pressure (AP) was directly recorded in conscious animals for 60 min. Square pulses (1 ms, 3 V, 30 Hz) were applied intermittently (20/20 s ON/OFF) to the CS for 48 h. After the end of stimulation, AP was recorded again. Nonstimulated rats (control group) and rats without electrodes around the CS (sham-operated) were also studied. Next, the animals were decapitated, and segments of mesenteric resistance arteries were removed to study vascular function. After the stimulation period, AP was 16 ± 5 mmHg lower in the stimulated group, whereas sham-operated and control rats showed similar AP between the first and second recording periods. Heart rate variability (HRV) evaluated using time and frequency domain tools and a nonlinear approach (symbolic analysis) suggested that hypertensive rats with electrodes around the CS, stimulated or not, exhibited a shift in cardiac sympathovagal balance towards parasympathetic tone. The relaxation response to acetylcholine in endothelium-intact mesenteric arteries was enhanced in rats that underwent CS stimulation for 48 h. In conclusion, long-term CS stimulation is effective in reducing AP levels, improving HRV and increasing mesenteric vascular relaxation in L-NAME hypertensive rats. Moreover, only the presence of electrodes around the CS is effective in eliciting changes in HRV similar to those observed in stimulated rats.