KCNQ1 Antibodies for Immunotherapy of Long QT Syndrome Type 2

• Published in: Journal of the American College of Cardiology 2020-05, Vol.75 (17), p.2140-2152
• Main Authors: Maguy, Ange, Kucera, Jan P., Wepfer, Jonas P., Forest, Virginie, Charpentier, Flavien, Li, Jin
• Format: Article
• Language: English
• Subjects: Animals; Autoantibodies - blood; cardiac arrhythmias; Cells, Cultured; CHO Cells; Cricetinae; Cricetulus; HEK293 Cells; Humans; IKs; immunotherapy; Immunotherapy - methods; KCNQ1 antibody; KCNQ1 Potassium Channel - blood; KCNQ1 Potassium Channel - chemistry; KCNQ1 Potassium Channel - immunology; long QT syndrome; Long QT Syndrome - blood; Long QT Syndrome - immunology; Long QT Syndrome - therapy; Membrane Potentials - physiology; Myocytes, Cardiac - immunology; Myocytes, Cardiac - metabolism; Proof of Concept Study; Protein Structure, Secondary; Rabbits
• ISSN: 0735-1097; 1558-3597
• DOI: 10.1016/j.jacc.2020.02.067

Patients with long QT syndrome (LQTS) are predisposed to life-threatening arrhythmias. A delay in cardiac repolarization is characteristic of the disease. Pharmacotherapy, implantable cardioverter-defibrillators, and left cardiac sympathetic denervation are part of the current treatment options, but no targeted therapy for LQTS exists to date. Previous studies indicate that induced autoimmunity against the voltage-gated KCNQ1 K+ channels accelerates cardiac repolarization. However, a causative relationship between KCNQ1 antibodies and the observed electrophysiological effects has never been demonstrated, and thus presents the aim of this study. The authors purified KCNQ1 antibodies and performed whole-cell patch clamp experiments as well as single-channel recordings on Chinese hamster ovary cells overexpressing IKs channels. The effect of purified KCNQ1 antibodies on human cardiomyocytes derived from induced pluripotent stem cells was then studied. The study demonstrated that KCNQ1 antibodies underlie the previously observed increase in repolarizing IKs current. The antibodies shift the voltage dependence of activation and slow the deactivation of IKs. At the single-channel level, KCNQ1 antibodies increase the open time and probability of the channel. In models of LQTS type 2 (LQTS2) using human induced pluripotent stem cell-derived cardiomyocytes, KCNQ1 antibodies reverse the prolonged cardiac repolarization and abolish arrhythmic activities. Here, the authors provide the first direct evidence that KCNQ1 antibodies act as agonists on IKs channels. Moreover, KCNQ1 antibodies were able to restore alterations in cardiac repolarization and most importantly to suppress arrhythmias in LQTS2. KCNQ1 antibody therapy may thus present a novel promising therapeutic approach for LQTS2. [Display omitted]