Phyllanthus fraternus manifests potent anti-proliferative activity on cultured Daudi cells

Objective: The aim of this study is to observe the apoptosis of Phyllanthus fraternus Webster against Daudi cells and to study its primary mechanism. Materials and Methods: Antiproliferative activity of cultured Daudi cells was evaluated using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium b...

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Published in:Journal of cancer research and therapeutics 2020-01, Vol.16 (1), p.71-77
Main Authors: Parmar, Felisa, George, Linz-Buoy, Highland, Hyacinth
Format: Article
Language:English
Subjects:
Analysis
Antineoplastic Agents, Phytogenic - pharmacology
Apoptosis
Bromine compounds
Cancer therapies
Cell culture
Cell Line, Tumor
Cell Proliferation
Cytotoxicity
Deoxyribonucleic acid
DNA
Electrophoresis
Ethanol
Genetic research
Herbal medicine
Humans
Isolation
Neoplasms - drug therapy
Neoplasms - metabolism
Neoplasms - pathology
Phyllanthus - chemistry
Plant Extracts - pharmacology
Proteins
Time
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Summary:Objective: The aim of this study is to observe the apoptosis of Phyllanthus fraternus Webster against Daudi cells and to study its primary mechanism. Materials and Methods: Antiproliferative activity of cultured Daudi cells was evaluated using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay in a dose- and time-dependent manner after treatment with the hydroalcoholic extract of P. fraternus . Trypan blue viability assay was also performed. Apoptosis induction in the cells posttreatment was determined by DNA fragmentation assay, Agarose gel electrophoresis, and Acridine orange/Ethidium bromide dual staining. Protein isolation and analysis was carried out using the standard polyacrylamide gel electrophoresis protocols. Results: The extracts inhibited the growth and proliferation of Daudi cells through induced cell death, which was dose-dependent and time-dependent. The IC50 value was found to be 220 μg/ml after 72 h of treatment. The induction of DNA fragmentation and increase in a number of apoptotic cells posttreatment suggest the possibility of apoptosis induction. A significant decrease in protein level was also observed. Conclusion: The results raise the possibility that the hydroalcoholic extract of P. fraternus could be a potent chemotherapeutic agent for the treatment of various cancers. Further evaluation of its potency as a chemotherapeutic agent is imperative.
ISSN:0973-1482
1998-4138
DOI:10.4103/jcrt.JCRT_429_17