Serum proteomics analysis reveals the thermal fitness of crossbred dairy buffalo to chronic heat stress

Chronic heat stress (CHS) reduces the production efficiency of the buffalo dairy industry. Relatively low-abundance proteins with particular functions in biological processes are changed by CHS. The present study aimed to quantify the differences in low-abundance proteins of crossbred dairy buffaloe...

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Bibliographic Details
Published in:Journal of thermal biology 2020-04, Vol.89, p.102547-8, Article 102547
Main Authors: Liu, Ping, Guo, Lulu, Mao, Huaming, Gu, Zhaobing
Format: Article
Language:English
Subjects:
Animals
Antimicrobial Cationic Peptides - blood
Buffaloes - blood
Buffaloes - genetics
Buffaloes - physiology
Ceruloplasmin
Ceruloplasmin - metabolism
Chronic heat stress
Dairy buffaloes
Dairy industry
Fitness
Glutathione peroxidase
Glutathione Peroxidase - blood
Heat stress
Hemoglobin
Hemoglobins - metabolism
Hybridization, Genetic
Hypoxia
Lipase
Lipase - blood
Oxygen Consumption
Proteins
Proteome - genetics
Proteome - metabolism
Proteomics
Thermal fitness
Thermotolerance
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Summary:Chronic heat stress (CHS) reduces the production efficiency of the buffalo dairy industry. Relatively low-abundance proteins with particular functions in biological processes are changed by CHS. The present study aimed to quantify the differences in low-abundance proteins of crossbred dairy buffaloes under CHS and thermal-neutral (TN) conditions. With label-free quantification, 344 low-abundance proteins were identified in serum. Of these, 17 differentially expressed low-abundance proteins with known functions were detected, and six of the differentially expressed proteins related to heat stress were validated with parallel reaction monitoring. Lipase (LPL), glutathione peroxidase 3 (GPX3), cathelicidin-2 (CATHL2), ceruloplasmin (CP), and hemoglobin subunit alpha 1 (HBA1) cooperatively played roles in the thermal fitness of dairy buffalo by decreasing heat production and increasing blood oxygen delivery. Also, dairy buffaloes may adapt to CHS and hypoxia with high levels of RBCs, HBA1 and CP to increase blood oxygen delivery capacity. •Heat stress causes oxidative stress and animal tissue hypoxia.•Hemoglobin and ceruloplasmin increase with heat stress to maintain a normal oxygen supply.•Glutathione peroxidase 3 and ceruloplasmin protect the cells of dairy buffalo from oxidative stress damage.•Lipase, glutathione peroxidase 3, ceruloplasmin, and hemoglobin subunit alpha 1 act together to increase the thermotolerance of dairy buffalo.
ISSN:0306-4565
1879-0992
DOI:10.1016/j.jtherbio.2020.102547