Inhibin A regulates follicular development via hormone secretion and granulosa cell behaviors in laying hens

Inhibin A regulates follicular development, and its expression level is related to physiological activities, such as the recruitment, selection, and predominance during follicular development. Therefore, examining inhibin A and its regulatory effects on the reproductive performance of poultry is cru...

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Published in:Cell and tissue research 2020-08, Vol.381 (2), p.337-350
Main Authors: Cui, Zhifu, Liu, Lingbin, Zhu, Qing, Wang, Yan, Yin, Huadong, Li, Diyan, Tian, Yaofu, Shu, Gang, Zhao, Xiaoling
Format: Article
Language:English
Subjects:
17β-Estradiol
Apoptosis
Bax protein
Bcl-2 protein
Biomedical and Life Sciences
Biomedicine
Caspase-3
Estradiol
Flow cytometry
Follicles
Gene expression
Granulosa cells
Human Genetics
Inhibin
Mitochondria
Molecular Medicine
Progesterone
Proliferating cell nuclear antigen
Proteins
Proteomics
Regular Article
Reproductive system
RNA
RNA-mediated interference
Secretion
siRNA
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description Inhibin A regulates follicular development, and its expression level is related to physiological activities, such as the recruitment, selection, and predominance during follicular development. Therefore, examining inhibin A and its regulatory effects on the reproductive performance of poultry is crucial. In this study, we measured the mRNA and protein abundances of INHA and INHBA in the chicken reproductive system and determined the hormone secretion and apoptosis of follicular granulosa cells (GCs) after being treated with inhibin A protein, and flow cytometry was performed to analyze GC apoptosis in INHA -specific small RNA interference (siRNA). We detected that INHA and INHBA were mainly expressed in chicken follicles. The highest INHA mRNA abundance was found in the fifth largest preovulatory follicle (F5) ( P  
doi_str_mv 10.1007/s00441-020-03207-8
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Therefore, examining inhibin A and its regulatory effects on the reproductive performance of poultry is crucial. In this study, we measured the mRNA and protein abundances of INHA and INHBA in the chicken reproductive system and determined the hormone secretion and apoptosis of follicular granulosa cells (GCs) after being treated with inhibin A protein, and flow cytometry was performed to analyze GC apoptosis in INHA -specific small RNA interference (siRNA). We detected that INHA and INHBA were mainly expressed in chicken follicles. The highest INHA mRNA abundance was found in the fifth largest preovulatory follicle (F5) ( P  < 0.05). INHBA mRNA expression in the largest preovulatory follicle (F1) was significantly higher than those in other follicles ( P  < 0.05). Similar results were found for INHA and INHBA protein expression in those follicles ( P  < 0.05). Treatment with inhibin A protein increased the activity of GCs in a dose-dependent manner ( P  < 0.05), which was characterized by decreased gene expression of pro-apoptotic factors Bax and Caspase-3 ( P  < 0.05) and increased expression of proliferation genes Bcl-2 and PCNA ( P  < 0.05). Additionally, inhibin A significantly increased the secretion of progesterone and estradiol ( P  < 0.05). 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Therefore, examining inhibin A and its regulatory effects on the reproductive performance of poultry is crucial. In this study, we measured the mRNA and protein abundances of INHA and INHBA in the chicken reproductive system and determined the hormone secretion and apoptosis of follicular granulosa cells (GCs) after being treated with inhibin A protein, and flow cytometry was performed to analyze GC apoptosis in INHA -specific small RNA interference (siRNA). We detected that INHA and INHBA were mainly expressed in chicken follicles. The highest INHA mRNA abundance was found in the fifth largest preovulatory follicle (F5) ( P  < 0.05). INHBA mRNA expression in the largest preovulatory follicle (F1) was significantly higher than those in other follicles ( P  < 0.05). Similar results were found for INHA and INHBA protein expression in those follicles ( P  < 0.05). Treatment with inhibin A protein increased the activity of GCs in a dose-dependent manner ( P  < 0.05), which was characterized by decreased gene expression of pro-apoptotic factors Bax and Caspase-3 ( P  < 0.05) and increased expression of proliferation genes Bcl-2 and PCNA ( P  < 0.05). Additionally, inhibin A significantly increased the secretion of progesterone and estradiol ( P  < 0.05). RNAi-mediated knockdown of INHA increased apoptosis in GCs via a Caspase-3-dependent mitochondrial pathway.]]></abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>32377876</pmid><doi>10.1007/s00441-020-03207-8</doi><tpages>14</tpages></addata></record>
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1432-0878
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source Springer Nature
subjects 17β-Estradiol
Apoptosis
Bax protein
Bcl-2 protein
Biomedical and Life Sciences
Biomedicine
Caspase-3
Estradiol
Flow cytometry
Follicles
Gene expression
Granulosa cells
Human Genetics
Inhibin
Mitochondria
Molecular Medicine
Progesterone
Proliferating cell nuclear antigen
Proteins
Proteomics
Regular Article
Reproductive system
RNA
RNA-mediated interference
Secretion
siRNA
title Inhibin A regulates follicular development via hormone secretion and granulosa cell behaviors in laying hens
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