Lutzomyia evandroi in a New Area of Occurrence of Leishmaniasis

• Published in: Acta parasitologica 2020-09, Vol.65 (3), p.716-722
• Main Authors: Ubirajara Filho, Carlos Roberto Cruz, da Silva Sales, Kamila Gaudêncio, Lima, Thiago Antônio Rodrigues Freire, Dantas-Torres, Filipe, Alves, Leucio Câmara, de Carvalho, Gílcia Aparecida, Ramos, Rafael Antonio Nascimento
• Format: Article
• Language: English
• Subjects: Altitude; Animal Systematics/Taxonomy/Biogeography; Biomedical and Life Sciences; Biomedicine; Climatic conditions; Deoxyribonucleic acid; DNA; Ecology; Epidemiology; Females; Flies; Global positioning systems; GPS; High altitude; Insects; Invertebrates; Kernels; Leishmania; Leishmaniasis; Light traps; Lutzomyia; Medical Microbiology; Microbiology; Original Paper; Parasites; Parasitic diseases; Parasitology; Physical factors; Remote sensing; Satellite navigation systems; Spatial distribution; Statistical analysis; Vector-borne diseases; Vectors
• ISSN: 1230-2821; 1896-1851
• DOI: 10.2478/s11686-020-00215-0

Purpose Sand flies are vectors of medical and veterinary concern, responsible for the transmission of Leishmania parasites. These invertebrates are widely distributed throughout the world and their abundance and diversity is dependent on several biotic and physical factors. Based on the epidemiological importance of these insects, the aim of this study was to assess the sand fly fauna of a new area of occurrence of leishmaniasis. Methods From October 2018 to September 2019, samplings were performed using CDC light traps at six different collection points located in a high altitude area in the municipality of Garanhuns, Northeastern Brazil. Geographic coordinates were recorded using the satellite remote sensing Global Positioning System (GPS) Garmin Etrex20. The spatial distribution of sand flies was evaluated using kernel density estimation (KDE). Engorged females ( n  = 12) were molecularly processed to search for Leishmania DNA. Results A total of 138 sand fly specimens were collected during the study period, with 100% (138/138) identified as Lutzomyia evandroi . Climatic conditions did not influence the number of sand flies (males and females) collected monthly, but a statistically significant difference was observed between the number of specimens and different collections points. The kernel map showed a heterogeneous spatial distribution of sand flies in the municipality of Garanhuns with a hotspot in the south of the region. Conclusion Data presented herein are pivotal in filling the gap on the epidemiology of leishmaniasis in this high altitude area, serving as an alert to local health authorities. It also indicated that the putative role of L. evandroi in the transmission of Leishmania parasites should be clarified.