Anticancer and antimetastatic activity of Hypomyces chrysospermus, a cosmopolitan parasite in different human cancer cells

• Published in: Molecular biology reports 2020-05, Vol.47 (5), p.3765-3778
• Main Authors: Dikmen, Miriş, Öztürk, Selin Engür, Cantürk, Zerrin, Ceylan, Göksu, Karaduman, Ayşe Betül, Yamaç, Mustafa
• Format: Article
• Language: English
• Subjects: A549 Cells - drug effects; Acetic acid; Animal Anatomy; Animal Biochemistry; Antineoplastic Agents - pharmacology; Antineoplastic Agents, Phytogenic - pharmacology; Antioxidants; Antioxidants - pharmacology; Apaf-1 protein; Apoptotic Protease-Activating Factor 1 - metabolism; Basidiomycota - pathogenicity; Biological activity; Biomedical and Life Sciences; Caco-2 Cells - drug effects; Cancer; Cell Line, Tumor; Cell Movement - drug effects; Cell Proliferation - drug effects; Cytotoxicity; Ethyl acetate; Histology; Humans; Hypocreales - metabolism; Hypocreales - physiology; Life Sciences; MCF-7 Cells - drug effects; Metastases; Morphology; mRNA; NF-kappa B - metabolism; NF-κB protein; Original Article; Parasites; Plant Extracts - pharmacology; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-020-05468-6

The importance of microbial natural compounds in drug research is increasing every year and they are used to prevent or treat a variety of diseases. Hypomyces chrysospermus is a cosmopolitan parasite of many Boletaceae members. Since not much work has been conducted to date, this study is undertaken to explore the anticancer effect, including the antiproliferative and antimetastatic activity of Hypomyces chrysospermus. The aim of this study is to determine the antiproliferative and antimetastatic activity of Hypomyces chrysospermus ethyl acetate extract, having antioxidant activity, against A549, Caco2, MCF-7 human cancer and CCD-19 Lu and CCD 841 CoN healthy human cell lines. Firstly, cytotoxic activity was determined by the WST-1 assay. After cell proliferations and anti-metastatic effects were investigated by a real-time cell analysis system (RTCA-DP) and IC 50 concentrations were calculated for each cell line. In addition, the expression levels of Apaf-1, TNF and NF-kB mRNA in cancer cells were investigated with RealTime-PCR. The ethyl acetate extract of Hypomyces chrysospermus presented anticancer activities including antiproliferative and antimetastatic effects. Hypomyces chrysospermus as a source of biologically active metabolites can be used as an important resource in the development of new anticancer effective agents.