Misidentification of Cutibacterium namnetense as Cutibacterium acnes among clinical isolates by MALDI-TOF VitekMS: usefulness of gyrB sequencing and new player in bone infections

The taxonomy modification of Propionibacterium sp. with the description of new species, especially Cutibacterium namnetense , raises the question of species distribution in routine clinical samples. We performed a retrospective study during 3 years before the implementation of MALDI-TOF. Two hundred...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 2020-08, Vol.39 (8), p.1605-1610
Main Authors: Ruffier d’Epenoux, L., Arshad, N., Bémer, P., Juvin, M-E., Le Gargasson, G., Guillouzouic, A., Corvec, S.
Format: Article
Language:English
Subjects:
Bacterial Typing Techniques
Biomedical and Life Sciences
Biomedicine
Bone Diseases, Infectious - epidemiology
Bone Diseases, Infectious - microbiology
Brief Report
Clinical isolates
DNA, Bacterial - analysis
France - epidemiology
Geographical distribution
Gram-Positive Bacterial Infections - epidemiology
Gram-Positive Bacterial Infections - microbiology
Humans
Infections
Internal Medicine
Medical Microbiology
New species
Phylogeny
Propionibacterium - classification
Propionibacterium - genetics
Propionibacterium - isolation & purification
Retrospective Studies
rRNA 16S
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Taxonomy
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Summary:The taxonomy modification of Propionibacterium sp. with the description of new species, especially Cutibacterium namnetense , raises the question of species distribution in routine clinical samples. We performed a retrospective study during 3 years before the implementation of MALDI-TOF. Two hundred sixty-nine isolates were included in the study. MALDI-TOF identification, 16S rRNA, and new developed gyrB partial sequencings were performed. The most representative species was C. acnes in 88% of the cases, regardless of the origin of the clinical sample. Eventually, we identified three C. namnetense strains, representing a 1.1% prevalence over the period of time, including two bone infections. MALDI-TOF databases should be regularly updated to incorporate new species. gyrB sequencing constitutes a both easy and relevant method to identify Cutibacterium sp. especially C. namnetense , a new player in bone infections.
ISSN:0934-9723
1435-4373
DOI:10.1007/s10096-020-03873-0