MicroRNA-127-3p regulates myoblast proliferation by targeting Sept7

Objective MicroRNAs (miRNAs) are highly conserved, endogenous small RNAs that regulate gene expression at the post-transcriptional level. miR-127 plays an essential role in myogenic differentiation in vivo and in vitro. However, it is not clear whether miR-127-3p affects myogenic cell proliferation....

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Bibliographic Details
Published in:Biotechnology letters 2020-09, Vol.42 (9), p.1633-1644
Main Authors: Li, Jie, Wang, Gaofu, Jiang, Jing, Zhang, Li, Zhou, Peng, Ren, Hangxing
Format: Article
Language:English
Subjects:
Animals
Applied Microbiology
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cell Line
Cell lines
Cell proliferation
Cell Proliferation - physiology
Chromosome 3
Flow cytometry
Gene expression
Kinases
Life Sciences
Mice
Microbiology
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
Muscles
Myoblasts
Myoblasts - metabolism
Original Research Paper
Post-transcription
Regulatory mechanisms (biology)
RNA-mediated interference
Septins - genetics
Septins - metabolism
Western blotting
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Summary:Objective MicroRNAs (miRNAs) are highly conserved, endogenous small RNAs that regulate gene expression at the post-transcriptional level. miR-127 plays an essential role in myogenic differentiation in vivo and in vitro. However, it is not clear whether miR-127-3p affects myogenic cell proliferation. Methods The detailed function of miR-127-3p in proliferative C2C12 cell lines and further identified its regulatory mechanism by qRT-PCR, western blot, flow cytometry analysis and luciferase reporter assay. Results Overexpression of miR-127-3p significantly inhibited proliferation of C2C12 cells and vice versa. Sept7 was a target gene of miR-127-3p using dual-luciferase reporter assay, qRT-PCR, and western blotting. The RNA interference analysis, in which Sept7 was downregulated, showed that Sept7 significantly promoted the proliferation of C2C12 cells. Besides, the expression level of Sept7 was detected analysis in muscle cells and tissues. Conclusions These findings reveal that miR-127-3p regulates myoblast proliferation by targeting Sept7.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-020-02906-0