Decrease of Rab11 prevents the correct dendritic arborization, synaptic plasticity and spatial memory formation

Emerging evidence shows that Rab11 recycling endosomes (REs Rab11) are essential for several neuronal processes, including the proper functioning of growth cones, synapse architecture regulation and neuronal migration. However, several aspects of REs Rab11 remain unclear, such as its sub-cellular di...

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Published in:Biochimica et biophysica acta. Molecular cell research 2020-09, Vol.1867 (9), p.118735-118735, Article 118735
Main Authors: Siri, Sebastian O., Rozés-Salvador, Victoria, de la Villarmois, Emilce Artur, Ghersi, Marisa S., Quassollo, Gonzalo, Pérez, Mariela F., Conde, Cecilia
Format: Article
Language:English
Subjects:
Animals
Dendrites - metabolism
Dentate Gyrus - physiology
Electrophysiological Phenomena
Female
Hippocampus - physiology
Long-Term Potentiation
Male
Neuronal development
Neuronal Plasticity
Neurons - physiology
Pregnancy
rab GTP-Binding Proteins - genetics
rab GTP-Binding Proteins - metabolism
Rab11
Rats
Recycling endosomes
Spatial Memory
Synaptic plasticity
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Summary:Emerging evidence shows that Rab11 recycling endosomes (REs Rab11) are essential for several neuronal processes, including the proper functioning of growth cones, synapse architecture regulation and neuronal migration. However, several aspects of REs Rab11 remain unclear, such as its sub-cellular distribution across neuronal development, contribution to dendritic tree organization and its consequences in memory formation. In this work we show a spatio-temporal correlation between the endogenous localization of REs Rab11 and developmental stage of neurons. Furthermore, Rab11-suppressed neurons showed an increase on dendritic branching (without altering total dendritic length) and misdistribution of dendritic proteins in cultured neurons. In addition, suppression of Rab11 in adult rat brains in vivo (by expressing shRab11 through lentiviral infection), showed a decrease on both the sensitivity to induce long-term potentiation and hippocampal-dependent memory acquisition. Taken together, our results suggest that REs Rab11 expression is required for a proper dendritic architecture and branching, controlling key aspects of synaptic plasticity and spatial memory formation. •Differential and dynamic distribution of recycling endosomes Rab11 in neurons•Rab11 participates in the proper branching of the dendritic tree.•Rab11 is necessary for specific delivery of proteins to the somatodendritic domain.•LTP and spatial memory are abolished after Rab11 knockdown in vivo.
ISSN:0167-4889
1879-2596
DOI:10.1016/j.bbamcr.2020.118735