Assessment of Embryonic Bioactivity through Changes in the Water Structure Using Near-Infrared Spectroscopy and Imaging

We explored the influence of embryonic bioactivity on the water structure using near-infrared (NIR) spectroscopy and imaging. Four groups of Japanese medaka fish ( ) eggs were studied: (a) one group of eggs was activated by fertilization, and (b-d) three groups of eggs were not activated because emb...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2020-06, Vol.92 (12), p.8133-8141
Main Authors: Ishigaki, Mika, Yasui, Yui, Kajita, Misato, Ozaki, Yukihiro
Format: Article
Language:English
Subjects:
Analytical chemistry
Animals
Biological activity
Bonding strength
Chemistry
Egg Yolk - chemistry
Egg Yolk - metabolism
Eggs
Embryogenesis
Embryonic growth stage
Fertilization
Freezing
Hydrogen
Hydrogen Bonding
Hydrogen bonds
I.R. radiation
Infrared imaging
Infrared spectra
Infrared spectroscopy
Near infrared radiation
Oryzias - embryology
Oryzias latipes
Ovum - chemistry
Ovum - metabolism
Proteins
Spectroscopy, Near-Infrared
Spectrum analysis
Water - chemistry
Water - metabolism
Yolk
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We explored the influence of embryonic bioactivity on the water structure using near-infrared (NIR) spectroscopy and imaging. Four groups of Japanese medaka fish ( ) eggs were studied: (a) one group of eggs was activated by fertilization, and (b-d) three groups of eggs were not activated because embryogenesis was stopped or not started by (b) culturing under cold temperature, (c) instant freezing, or (d) lack of fertilization. The yolks of the activated eggs contained higher proportions of weakly hydrogen bonded water than those of nonactivated eggs. A possible factor responsible for the significant changes in the water structure was revealed to be a protein secondary structural change from an α-helix to a β-sheet in the activated eggs. NIR images of the activated eggs successfully visualized the water structural variation in the yolk with a higher proportion of weak hydrogen bonds due to the activation of embryonic development. The embryogenic activity could be assessed through the water hydrogen bond network, which is affected by newly generated proteins with different secondary structures.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.0c00076