Effects of high-level expression of A1-ATPase on H2 production in Thermococcus kodakarensis

• Published in: Journal of bioscience and bioengineering 2020-08, Vol.130 (2), p.149-158
• Main Authors: Simons, Jan-Robert, Beppu, Haruki, Imanaka, Tadayuki, Kanai, Tamotsu, Atomi, Haruyuki
• Format: Article
• Language: English
• Subjects: A1-type ATPase; Archaea; Hydrogenase; Hyperthermophiles; Molecular hydrogen; Thermococcus kodakarensis
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2020.04.001

The hyperthermophilic archaeon Thermococcus kodakarensis can grow on pyruvate or maltooligosaccharides through H2 fermentation. H2 production levels of members of the Thermococcales are high, and studies to improve their production potential have been reported. Although H2 production is primary metabolism, here we aimed to partially uncouple cell growth and H2 production of T. kodakarensis. Additional A1-type ATPase genes were introduced into T. kodakarensis KU216 under the control of two promoters; the strong constitutive cell surface glycoprotein promoter, Pcsg, and the sugar-inducible fructose-1,6-bisphosphate aldolase promoter, Pfba. Whereas cells with the A1-type ATPase genes under the control of Pcsg displayed only trace levels of growth, cells with Pfba (strain KUA-PF) displayed growth sufficient for further analysis. Increased levels of A1-type ATPase protein were detected in KUA-PF cells grown on pyruvate or maltodextrin, when compared to the levels in the host strain KU216. The growth and H2 production levels of strain KUA-PF with pyruvate or maltodextrin as a carbon and electron source were analyzed and compared to those of the host strain KU216. Compared to a small decrease in total H2 production, significantly larger decreases in cell growth were observed, resulting in an increase in cell-specific H2 production. Quantification of the substrate also revealed that ATPase overexpression led to increased cell-specific pyruvate and maltodextrin consumptions. The results clearly indicate that ATPase production results in partial uncoupling of cell growth and H2 production in T. kodakarensis.