Diel pattern in the structure and function of the gut microbial community in Lymantria dispar asiatica (Lepidoptera: Lymantriidae) larvae

In the present study, diel pattern in gut microbial communities in insects were evaluated. Lymantria dispar asiatica fourth instar larvae (72 ± 2 hr after molting) at noon (LdD) and midnight (LdN) were used for a comparative analysis of the gut microbial community. Ten bacterial operational taxonomi...

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Bibliographic Details
Published in:Archives of insect biochemistry and physiology 2020-07, Vol.104 (3), p.e21691-n/a
Main Authors: Zeng, Jian‐Yong, Vuong, Thi‐Minh‐Dien, Guo, Jia‐Xing, Shi, Jiang‐Hong, Shi, Zhong‐Bin, Zhang, Guo‐Cai, Zhang, Jie
Format: Article
Language:English
Subjects:
Bacteria
Butterflies & moths
circadian
Comparative analysis
Discriminant analysis
gut microbial community
Insects
Intestinal microflora
Larvae
Lymantria dispar
Lymantria dispar asiatica larvae
Microbial activity
Microbiomes
Microbiota
Microorganisms
Molting
Phylogeny
Structure-function relationships
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Summary:In the present study, diel pattern in gut microbial communities in insects were evaluated. Lymantria dispar asiatica fourth instar larvae (72 ± 2 hr after molting) at noon (LdD) and midnight (LdN) were used for a comparative analysis of the gut microbial community. Ten bacterial operational taxonomic units (OTUs) were shared between LdD and LdN samples. One bacterial OTU was specific to LdD. The dominant gut microbes were OTU72 in LdD and OTU75 in LdN. A linear discriminant analysis effect size cladogram suggested that ten bacterial OTUs maintain significant differences in relative abundances between LdD and LdN. These results agreed with the discrete ellipses between LdD and LdN in principal coordinates analysis plots. Additionally, using phylogenetic investigation of communities by reconstruction of unobserved states, the gut microbial community was assigned to 23 functional terms, among which 22 exhibited significant differences between LdD and LdN. To conclude, the present study documented a diel pattern in the gut microbial community of L. dispar asiatica larvae. Comparative analysis of the gut microbial community in Lymantria dispar asiatica fourth instar larvae using linear discriminant analysis effect size cladogram. Experimental larvae were sampled at noon (LdD) and midnight (LdN) respectively. The linear discriminant analysis threshold was two. Research Highlight Diel pattern in gut microbiota in gypsy moth larvae were characterized
ISSN:0739-4462
1520-6327
DOI:10.1002/arch.21691