Molecular characterization of different preproGnRHs in Astyanax altiparanae (Characiformes): Effects of GnRH on female reproduction

Gonadotropin‐releasing hormone (GnRH) is a key molecule in the initiation of the hypothalamic–pituitary–gonadal axis. Thus, knowledge about GnRH may contribute to the effectiveness of species reproduction. Using a Neotropical tetra Astyanax altiparanae as a fish model species, the GnRH forms were ch...

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Published in:Molecular reproduction and development 2020-06, Vol.87 (6), p.720-734
Main Authors: Chehade, Chayrra, Amaral, Fernanda G., Branco, Giovana S., Cassel, Mônica, De Jesus, Lázaro W. O., Costa, Fabiano G., Bordin, Silvana A., Moreira, Renata G., Borella, Maria I.
Format: Article
Language:English
Subjects:
Amino acids
Astyanax altiparanae
Complementary DNA
fshβ expression
Gene expression
Gonadotropin-releasing hormone
Gonadotropins
hypothalamic–pituitary–gonadal axis
Hypothalamus
induced spawning
lhβ expression
maturation‐induced steroid levels
Nucleotide sequence
Pituitary
Pituitary (anterior)
Spawning
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Summary:Gonadotropin‐releasing hormone (GnRH) is a key molecule in the initiation of the hypothalamic–pituitary–gonadal axis. Thus, knowledge about GnRH may contribute to the effectiveness of species reproduction. Using a Neotropical tetra Astyanax altiparanae as a fish model species, the GnRH forms were characterized at the molecular level and the role of injected GnRHs in vivo was evaluated. The full‐length complementary DNA (cDNA) sequences of preproGnRH2 (612 bp) and preproGnRH3 (407 bp) of A. altiparanae were obtained, and the GnRH1 form was not detected. The cDNA sequences of preproGnRH2 and preproGnRH3 were found to be conserved, but a change in the amino acid at position 8 of the GnRH3 decapeptide of A. altiparanae was observed. All the injected GnRHs stimulated lhβ messenger RNA (mRNA) expression but not fshβ mRNA expression, and only GnRH2 was able to increase maturation‐inducing steroid (MIS) levels and possibly stimulate oocyte release. Furthermore, only GnRH2 was able to start the entire reproductive hormonal cascade and induce spawning. cDNA sequences of preproGnRH2 and preproGnRH3 in A. altiparanae are found to be conserved. All GnRH forms stimulated the lhβ but not the fshβ mRNA expression. Only GnRH2 was able to increase the MIS level and stimulate the release of gametes.
ISSN:1040-452X
1098-2795
DOI:10.1002/mrd.23351