Improved viability of Akkermansia muciniphila by encapsulation in spray dried succinate-grafted alginate doped with epigallocatechin-3-gallate

• Published in: International journal of biological macromolecules 2020-09, Vol.159, p.373-382
• Main Authors: Chang, Yifan, Yang, Yu, Xu, Ningning, Mu, Haibo, Zhang, Hongli, Duan, Jinyou
• Format: Article
• Language: English
• Subjects: Akkermansia - drug effects; Akkermansia muciniphila; Alginates - chemistry; Antioxidants - chemistry; Antioxidants - pharmacology; Capsules - chemistry; Catechin - analogs & derivatives; Catechin - chemistry; Coated Materials, Biocompatible - chemistry; Epigallocatechin gallate; Magnetic Resonance Spectroscopy; Microbial Viability - drug effects; Microgels - chemistry; Molecular Structure; Obesity; Oxidation-Reduction; Particle Size; Phosphatidylethanolamines - chemistry; Sodium alginate; Spectroscopy, Fourier Transform Infrared; Succinic Acid - chemistry; Succinic anhydride; Surface Properties
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2020.05.055

We explored the possibility of improving the viability of Akkermansia muciniphila by encapsulating it in succinate-grafted alginate doped with epigallocatechin-3-gallate (EGCG). In this study, the determined surface properties of microcapsules and modified materials and the measured viability of probiotics after spray drying showed that the modified sodium alginate made the surfaces of microcapsules smoother and denser during the spray drying, thus preventing damages. EGCG enhanced the antioxidant capacity of probiotics by filling the pores inside microgels. Moreover, we analyzed the long-term storage vitality changes, oxidation resistance, uniformity, particle size and Zeta potential of microcapsules and found that spray-dried modified sodium alginate microcapsules with EGCG showed the better storability and stability. In addition, we experimentally analyzed the resistances of different microcapsules to the gastrointestinal fluid and found that EGCG-modified sodium alginate microcapsules better protected the probiotic activity from gastrointestinal fluid. This study provides a slimming product with industrial application potential. •Muciniphila, which is difficult to culture and preserve, was embedded.•Succinic anhydride has the potential to treat obesity.•Microcapsules in article showed good performance in a series of activity tests.•Epigallocatechin gallate is a very effective natural antioxidant compound.