Macrophage migration inhibitory factor gene polymorphisms as exacerbating factors of apical periodontitis

Two polymorphisms in the macrophage migration inhibitory factor (MIF) gene have been associated with inflammatory diseases (-794 CATT5-8 and -173G>C); however, so far there are no reports of studies related to oral health. To genotype the -794 CATT5-8 and -173G>C MIF polymorphisms in Mexican p...

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Published in:Advances in clinical and experimental medicine : official organ Wroclaw Medical University 2020-05, Vol.29 (5), p.597-602
Main Authors: Freer-Rojas, Andrea, Martínez-Garibay, Luis Carlos, Torres-Méndez, Fernando, Dávila-Pérez, Claudia Edith, Martínez-Castañón, Gabriel Alejandro, Patiño-Marín, Nuria, Alegría-Torres, Jorge Alejandro
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Language:English
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Summary:Two polymorphisms in the macrophage migration inhibitory factor (MIF) gene have been associated with inflammatory diseases (-794 CATT5-8 and -173G>C); however, so far there are no reports of studies related to oral health. To genotype the -794 CATT5-8 and -173G>C MIF polymorphisms in Mexican patients with apical periodontitis as a genetic risk of exacerbation. The study involved 120 patients with apical periodontitis: 60 with a diagnosis of acute apical periodontitis (Group A) and 60 without previous episodes of exacerbation (Group B). Allelic discrimination was performed from peripheral blood DNA; the repeat polymorphism -794 CATT5-8 was genotyped with sequencing, while the -173G>C polymorphism was determined using real-time polymerase chain reaction (RT-PCR) using TaqMan probes. The associations between MIF polymorphisms, haplotypes and the risk of exacerbated apical periodontitis were assessed. The allele CATT7 was associated with the risk of a stage of acute inflammation (OR = 4.13; 95% CI = 1.82-9.63; p =< 0.001). Regarding the -173G >C polymorphism, a process of inflammation exacerbation was only associated with the CC genotype (OR = 4.1; 95% CI = 1.02-20.84; p = 0.045). The analysis of the haplotype showed that the combination CATT7/C increases the risk of exacerbation of apical periodontitis (OR = 3.57; 95% CI = 1.038-13.300; p = 0.021). The polymorphisms -794 CATT5-8 and -173G>C MIF seem to significantly influence the development of a state of exacerbated inflammation in patients with apical periodontitis.
ISSN:1899-5276
DOI:10.17219/acem/121508