Non-electrophilic TRPA1 agonists, menthol, carvacrol and clotrimazole, open epithelial tight junctions via TRPA1 activation

Abstract Activation of the transient receptor potential A1 channel (TRPA1) by electrophilic agonists was reported to induce the opening of tight junctions (TJs). Because compounds that increase TJ permeability can be paracellular permeability enhancers, we investigated the effect of non-electrophili...

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Published in:Journal of biochemistry (Tokyo) 2020-10, Vol.168 (4), p.407-415
Main Authors: Mukaiyama, Minagi, Usui, Takeo, Nagumo, Yoko
Format: Article
Language:English
Subjects:
Animals
Antifungal Agents - pharmacology
Antipruritics - pharmacology
Cells, Cultured
Clotrimazole - pharmacology
Cymenes - pharmacology
Dogs
Epithelial Cells - drug effects
Epithelial Cells - metabolism
Menthol - pharmacology
Tight Junctions - drug effects
Tight Junctions - metabolism
TRPA1 Cation Channel - agonists
TRPA1 Cation Channel - metabolism
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cited_by cdi_FETCH-LOGICAL-c368t-a48ef35c11e4df2ca20ac5068876944b4899912adf948cd94d326ad3718d75d23
cites cdi_FETCH-LOGICAL-c368t-a48ef35c11e4df2ca20ac5068876944b4899912adf948cd94d326ad3718d75d23
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container_issue 4
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container_title Journal of biochemistry (Tokyo)
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creator Mukaiyama, Minagi
Usui, Takeo
Nagumo, Yoko
description Abstract Activation of the transient receptor potential A1 channel (TRPA1) by electrophilic agonists was reported to induce the opening of tight junctions (TJs). Because compounds that increase TJ permeability can be paracellular permeability enhancers, we investigated the effect of non-electrophilic TRPA1 activators, including food ingredients (menthol and carvacrol) and medication (clotrimazole), on epithelial permeability. We show that all three compounds induced increase of the permeability of fluorescein isothiocyanate-conjugated dextran (4 kDa) and decrease of transepithelial electrical resistance, accompanied by Ca2+ influx and cofilin activation in epithelial MDCK II monolayers. These phenotypes were attenuated by pretreatment of a TRPA1 antagonist, suggesting TRPA1-mediated opening of TJs. These results suggest that non-electrophilic TRPA1 activators with established safety can be utilized to regulate epithelial barriers.
doi_str_mv 10.1093/jb/mvaa057
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subjects Animals
Antifungal Agents - pharmacology
Antipruritics - pharmacology
Cells, Cultured
Clotrimazole - pharmacology
Cymenes - pharmacology
Dogs
Epithelial Cells - drug effects
Epithelial Cells - metabolism
Menthol - pharmacology
Tight Junctions - drug effects
Tight Junctions - metabolism
TRPA1 Cation Channel - agonists
TRPA1 Cation Channel - metabolism
title Non-electrophilic TRPA1 agonists, menthol, carvacrol and clotrimazole, open epithelial tight junctions via TRPA1 activation
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