Effect of Ginkgo biloba extract on pacemaker channels encoded by HCN gene

Background In the present study, the electropharmacological activity of traditional Chinese medicine, Ginkgo biloba extract (GBE), on human hyperpolarization-activated nucleotide-gated (HCN) channels and the underlying “funny” currents was investigated. Methods Standard two-electrode voltage-clamp r...

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Bibliographic Details
Published in:Herz 2021-06, Vol.46 (3), p.255-261
Main Authors: Chen, Hui, Chen, Yongjun, Yang, Jing, Wu, Pan, Wang, Xin, Huang, Congxin
Format: Article
Language:English
Subjects:
Arrhythmia
Cardiology
Channels
Controlled conditions
Cyclic Nucleotide-Gated Cation Channels - genetics
Deactivation
Gametocytes
Ginkgo biloba
Herbal medicine
Humans
Hyperpolarization
Internal Medicine
Ion channels (cyclic nucleotide-gated)
Ions
Kinetics
Medicine
Medicine & Public Health
Nucleotides
Oocytes
Original Articles
Pacemakers
Patch-Clamp Techniques
Plant Extracts
Potassium Channels - genetics
Traditional Chinese medicine
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Summary:Background In the present study, the electropharmacological activity of traditional Chinese medicine, Ginkgo biloba extract (GBE), on human hyperpolarization-activated nucleotide-gated (HCN) channels and the underlying “funny” currents was investigated. Methods Standard two-electrode voltage-clamp recordings were employed to examine the properties of cloned HCN subunit currents expressed in Xenopus oocytes under controlled conditions and GBE administration. Results We found that GBE irreversibly inhibited the HCN2 and HCN4 channel currents in a concentration-dependent fashion and that the HCN4 current was more sensitive to GBE compared with HCN2. In addition, GBE inhibition of the current amplitudes of HCN2 and HCN4 currents was accompanied by a decrease in the activation and deactivation kinetics. Conclusion The results of this study contribute toward illustrating the antiarrhythmic mechanism of GBE, which might be useful for the treatment of arrhythmia.
ISSN:0340-9937
1615-6692
DOI:10.1007/s00059-020-04933-z