Identification of two novel Hessian fly resistance genes H35 and H36 in a hard winter wheat line SD06165

• Published in: Theoretical and applied genetics 2020-08, Vol.133 (8), p.2343-2353
• Main Authors: Zhao, Lanfei, Abdelsalam, Nader Ragab, Xu, Yunfeng, Chen, Ming-Shun, Feng, Yi, Kong, Lingrang, Bai, Guihua
• Format: Article
• Language: English
• Subjects: Agriculture; Alleles; Animals; Biochemistry; Biomedical and Life Sciences; Biotechnology; Chromosome 7; Chromosome Mapping; Cultivars; Diptera; Disease Resistance - genetics; Genes; Genes, Plant - genetics; Genetic aspects; Genetic Linkage; Genetic Markers; Genotype; Genotyping; Greenhouses; High-Throughput Nucleotide Sequencing; Inbreeding; Life Sciences; Mayetiola destructor; Original Article; Pest resistance; Pests; Phenotype; Phenotypic variations; Plant Biochemistry; Plant breeding; Plant Breeding/Biotechnology; Plant Diseases - genetics; Plant Diseases - parasitology; Plant Genetics and Genomics; Plants, Genetically Modified; Polymerase chain reaction; Polymorphism, Single Nucleotide; Quantitative genetics; Quantitative Trait Loci; Single nucleotide polymorphisms; Single-nucleotide polymorphism; Triticum - genetics; Triticum - parasitology; Triticum aestivum; Wheat
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-020-03602-3

Key message Two new Hessian fly resistance QTLs ( H35 and H36 ) and tightly linked SNP markers were identified in a US hard winter wheat SD06165. Hessian fly (HF), Mayetiola destructor (Say), is one of the most destructive pests in wheat ( Triticum aestivum L.) worldwide. Growing resistant cultivars is the most effective approach to minimize Hessian fly damage. To identify new quantitative trait loci (QTLs) for HF resistance, a recombinant inbred line population was developed by crossing HF resistant wheat line SD06165 to a susceptible line OK05312. The population was genotyped with 1709 single-nucleotide polymorphisms (SNPs) generated from genotyping-by-sequencing and phenotyped for HF resistance in greenhouses. Two novel QTLs for HF resistance were identified from SD06165. The major QTL, designated as H35, was closely linked to SNP marker SDOKSNP7679 on chromosome 3BS that explained 23.8% and 36.0% of the phenotypic variations; the minor QTL, designated as H36 , was flanked by SNP markers SDOKSNP1618 and SDOKSNP8089 on chromosome 7AS and explained 8.5% and 13.1% of the phenotypic variation in the two experiments. Significant interaction was detected between the two QTLs. Seventeen SNPs that tightly link to H35 and eight SNPs that tightly link to H36 were converted to kompetitive allele specific polymerase chain reaction markers for selecting these QTLs in breeding programs.