Fluoxetine administration in juvenile overfed rats improves hypothalamic mitochondrial respiration and REDOX status and induces mitochondrial biogenesis transcriptional expression

• Published in: European journal of pharmacology 2020-08, Vol.881, p.173200-173200, Article 173200
• Main Authors: Braz, Glauber Rudá Feitoza, Silva, Severina Cassia de Andrade, Pedroza, Anderson Apolonio da Silva, de Lemos, Maria Daniele, de Lima, Flávia Ariane, da Silva, Aline Isabel, Lagranha, Claudia Jacques
• Format: Article
• Language: English
• Subjects: Age Factors; Animal Nutritional Physiological Phenomena; Animals; Animals, Newborn; Animals, Suckling; Anti-Obesity Agents - pharmacology; Electron transport chain complex proteins; Energy Metabolism - drug effects; Fluoxetine - pharmacology; Hypothalamus - drug effects; Hypothalamus - metabolism; Hypothalamus - pathology; Hypothalamus - physiopathology; Male; Mitochondria - drug effects; Mitochondria - genetics; Mitochondria - metabolism; Mitochondria - pathology; Nutritional Status; Organelle Biogenesis; Overfeeding; Overnutrition - drug therapy; Overnutrition - metabolism; Overnutrition - pathology; Overnutrition - physiopathology; Oxidation-Reduction; Oxidative phosphorylation; Oxidative stress; Oxidative Stress - drug effects; Oxygen Consumption; Rats, Wistar; Reactive Oxygen Species - metabolism; RT-PCR; Serotonin reuptake inhibitor; Transcription, Genetic; Uncoupling Protein 2 - genetics; Uncoupling Protein 2 - metabolism
• ISSN: 0014-2999; 1879-0712
• DOI: 10.1016/j.ejphar.2020.173200

Nutritional imbalance in early life may disrupt the hypothalamic control of energy homeostasis and increase the risk of metabolic disease. The hypothalamic serotonin (5-hydroxytryptamine; 5-HT) system based in the hypothalamus plays an important role in the homeostatic control of energy balance, however the mechanisms underlying the regulation of energy metabolism by 5-HT remain poorly described. Several crucial mitochondrial functions are altered by mitochondrial stress. Adaptations to this stress include changes in mitochondrial multiplication (i.e, mitochondrial biogenesis). Due to the scarcity of evidence regarding the effects of serotonin reuptake inhibitors (SSRI) such as fluoxetine (FLX) on mitochondrial function, we sought to investigate the potential contribution of FLX on changes in mitochondrial function and biogenesis occurring in overfed rats. Using a neonatal overfeeding model, male Wistar rats were divided into 4 groups between 39 and 59 days of age based on nutrition and FLX administration: normofed + vehicle (NV), normofed + FLX (NF), overfed + vehicle (OV) and overfed + FLX (OF). We found that neonatal overfeeding impaired mitochondrial respiration and increased oxidative stress biomarkers in the hypothalamus. FLX administration in overfed rats reestablished mitochondrial oxygen consumption, increased mitochondrial uncoupling protein 2 (Ucp2) expression, reduced total reactive species (RS) production and oxidative stress biomarkers, and up-regulated mitochondrial biogenesis-related genes. Taken together our results suggest that FLX administration in overfed rats improves mitochondrial respiratory chain activity and oxidative balance and increases the transcription of genes employed in mitochondrial biogenesis favoring mitochondrial energy efficiency in response to early nutritional imbalance.