Transcriptomic and biochemical analyses of the accumulation of sucrose in mungbean (Vigna radiata (L.) Wilczek) leaves after pod removal

• Published in: Theoretical and applied genetics 2020-08, Vol.133 (8), p.2355-2362
• Main Authors: Ha, Jungmin, Shim, Sangrea, Lee, Taeyoung, Lee, Eunsoo, Yang, Xuefei, Jeong, Haneul, Kim, Moon Young, Lee, Suk-Ha
• Format: Article
• Language: English
• Subjects: Agriculture; Beans; Biochemistry; Biomedical and Life Sciences; Biotechnology; Chromatography, High Pressure Liquid; Cultivars; Gene expression; Gene Expression Regulation, Plant - genetics; Gene Ontology; Genotype; Genotypes; High-performance liquid chromatography; Leaves; Life Sciences; Maturity; Original Article; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant Leaves - genetics; Plant Leaves - metabolism; Protein sources; Real-Time Polymerase Chain Reaction; Ribonucleic acid; RNA; RNA-Seq; Seeds - genetics; Seeds - growth & development; Seeds - metabolism; Signal Transduction - genetics; Starch - genetics; Starch - metabolism; Sucrose; Sucrose - metabolism; Transcriptome - genetics; Vigna - genetics; Vigna - growth & development; Vigna - metabolism; Vigna radiata; β-Glucosidase
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-020-03603-2

Key message Synchronous pod maturity is critical for increasing grain yield. The candidate genes involved in synchronous pod maturity were identified through RNA-seq and HPLC. Mungbean ( Vigna radiata [L.] Wilczek), an important source of carbohydrate and protein in Asia, is characterized by nonsynchronous pod maturity; consequently, harvesting is labor intensive. Because pod maturity is associated with synthesis and remobilization of sucrose, we examined changes in sucrose levels and transcriptome in leaf (source) tissues after pod (sink) removal using two genotypes, VC1973A and V2984; VC1973A had higher synchronicity in pod maturity than V2984. After pod removal, much higher number of pods were produced in V2984 than VC1973A. The sucrose content of leaf tissues significantly decreased in V2984 because it continued to utilize assimilates from leaves for producing new pods, but significantly increased in VC1973A because of the loss of sink. Transcriptome analysis revealed that the number of differentially expressed genes was approximately fourfold higher in VC1973A than in those of V2984 after pod removal. The expression of two paralogous genes ( Vradi01g05010 and Vradi10g08240 ), encoding beta-glucosidase enzymes, significantly decreased in VC1973A after pod removal and was significantly lower in depodded VC1973A than depodded V2984, indicating these two genes may participate in sucrose utilization for seed development by regulating the level of glucose. The results of this study will help elucidate the genetic basis of synchronous pod maturity in mungbean and facilitate the development of new cultivars with synchronous pod maturity.