Effect of graphene oxide with different morphological characteristics on properties of immobilized enzyme in the covalent method

Although graphene oxide (GO) has great potential in the field of immobilized enzyme catalysts, the detailed effects of GO with different morphological structures on immobilized enzyme are not well understood. GOs were prepared from 8000 mesh and nanoscale graphite at different reaction temperatures,...

Full description

Saved in:
Bibliographic Details
Published in:Bioprocess and biosystems engineering 2020-10, Vol.43 (10), p.1847-1858
Main Authors: Zhang, Han, Hua, Shao-Feng, Li, Cong-qiang, Zhang, Lei, Fan, Yun-Chang, Duan, Peigao
Format: Article
Language:English
Subjects:
Amylases
Atomic force microscopes
Atomic force microscopy
Biotechnology
Catalysts
Chemistry
Chemistry and Materials Science
Crosslinking
Environmental Engineering/Biotechnology
Enzymes
Food Science
Graphene
Immobilization
Immobilized enzymes
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Morphology
pH effects
Photoelectron spectroscopy
Photoelectrons
Physical characteristics
Research Paper
Shelf life
Silicon
Storage stability
α-Amylase
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Although graphene oxide (GO) has great potential in the field of immobilized enzyme catalysts, the detailed effects of GO with different morphological structures on immobilized enzyme are not well understood. GOs were prepared from 8000 mesh and nanoscale graphite at different reaction temperatures, and used as carriers to immobilize alpha-amylase by cross-linking method. The properties of GOs were characterized through Atomic force microscope, Fourier-transformed infrared, X-ray photoelectron spectroscopy, Raman and UV–Vis. Furthermore, the dosage of cross-linking agent, cross-linking time, optimum temperature/pH, thermal/pH/storage stability, reusability and kinetic parameters of immobilized enzymes were investigated. The results showed that the loading of alpha-amylase on GOs was 162.3–274.2 mg g −1 . The reusability experiments revealed high activity maintenance of immobilized alpha-amylase even after seven reaction cycles. Moreover, the storage stability of immobilized enzyme improved via immobilization in comparison with free one and it maintained over 70% of their initial activity after 20 days storage at 4 °C.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-020-02375-9