Development of a highly sensitive liquid chromatography–mass spectrometry method to quantify plasma leukotriene E4 and demonstrate pharmacological suppression of endogenous 5-LO pathway activity in man

•A robust ultra-sensitive SPE-UPLC-MS/MS method for LTE4 in human plasma was successfully developed•For the first time we demonstrated that pharmacological inhibition of 5-LO pathway activity can suppress basal endogenous levels of LTE4 in plasma•The ability to quantify plasma LTE4 provides an alter...

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Bibliographic Details
Published in:Prostaglandins & other lipid mediators 2020-10, Vol.150, p.106463-106463, Article 106463
Main Authors: Löfgren, Lars, Forsberg, Gun-Britt, Davidsson, Pia, Eketjäll, Susanna, Whatling, Carl
Format: Article
Language:English
Subjects:
5-lipoxygenase
AZD5718
Cardiovascular disease
FLAP
Leukotriene E4
Liquid chromatography
LTE4
Mass spectrometry
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Summary:•A robust ultra-sensitive SPE-UPLC-MS/MS method for LTE4 in human plasma was successfully developed•For the first time we demonstrated that pharmacological inhibition of 5-LO pathway activity can suppress basal endogenous levels of LTE4 in plasma•The ability to quantify plasma LTE4 provides an alternative biomarker to urine LTE4 to assess endogenous 5-LO pathway activity in man. Low basal endogenous concentrations (
ISSN:1098-8823
DOI:10.1016/j.prostaglandins.2020.106463