Interleukin‐13 induces paralysis of Haemonchus contortus larvae in vitro

• Published in: Parasite immunology 2020-10, Vol.42 (10), p.e12758-n/a
• Main Authors: Shepherd, Elizabeth, Greiner, Scott P., Russ, Brynnan, Bowdridge, Scott A.
• Format: Article
• Language: English
• Subjects: Animals; Blocking antibodies; Contractility; Cytokines; Gastrointestinal tract; Haemonchiasis - immunology; Haemonchiasis - veterinary; Haemonchus - drug effects; Haemonchus - immunology; Haemonchus contortus; helminth; Immune response; immunity; Interleukin-13 - pharmacology; Interleukin‐13; Larva - drug effects; Larva - immunology; Larvae; Levamisole; Lymphocytes T; Monocytes; Morbidity; Motility; ovine; Paralysis; Sheep; Sheep Diseases - diet therapy; Sheep Diseases - parasitology; Sheep, Domestic
• ISSN: 0141-9838; 1365-3024
• DOI: 10.1111/pim.12758

Aims Interleukin‐13 (IL‐13) is a Th2‐associated cytokine that typically induces gut contractility and mucus secretion to eliminate helminth parasites from the digestive tract. Little evidence exists of IL‐13's direct effect on Haemonchus contortus larvae (L3) and thus was the objective of this study. Methods To test effects of IL‐13 on H contortus, L3 were treated with ovine recombinant (r) IL‐13 (1 μg/mL); motility and morbidity were assessed. Monocytes isolated from H contortus‐resistant St. Croix (STC) and susceptible Suffolk (SUF) sheep were treated with anti–IL‐13 blocking antibody to elucidate differences in host immune response. Results rIL‐13 treatment reduced L3 speed (27 μm/s) and distance (7.5 μm) compared to untreated L3 (speed: 94 μm/s; distance: 27 μm) (P