Taoren-dahuang herb pair reduces eicosanoid metabolite shifts by regulating ADORA2A degradation activity in ischaemia/reperfusion injury rats

• Published in: Journal of ethnopharmacology 2020-10, Vol.260, p.113014-113014, Article 113014
• Main Authors: Li, Liu-Liu, Liu, Yan-Ru, Sun, Chen, Yan, Yong-Gang, Tang, Zhi-Shu, Sun, Jing, Li, Lu-Han, Song, Zhong-Xing, Wang, Dan-Yang, Li, Xiao-Hong, Chang, Ai-Bing, Yan, Ya-Feng, Gao, Jing, Peng, Liang
• Format: Article
• Language: English
• Subjects: Animals; Brain - drug effects; Brain - pathology; Cerebral ischaemia/reperfusion injury; Drugs, Chinese Herbal - pharmacology; Eicosanoids - metabolism; Inflammation - drug therapy; Inflammation - pathology; Male; Medicine, Chinese Traditional; Plant Roots; Potential mechanism; Protective effects; Prunus - chemistry; Rats; Rats, Sprague-Dawley; Receptor, Adenosine A2A - metabolism; Reperfusion Injury - drug therapy; Reperfusion Injury - physiopathology; Rheum - chemistry; Rhizome; Seeds; TD herb Pair
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2020.113014

Peach kernel (taoren: TR) is the dried mature seed of peach, Prunus persica (L.) Batsch, which belongs to the Rosaceae family. Rhubarb (dahuang: DH) is the dried root and rhizome of rhubarb (Rheum palmatum L., Rheum officinale Baill., or Rheum tanguticum Maxim. ex Balf.). TR-DH (TD) is a traditional Chinese medicine herb pair that promotes blood circulation and removes blood stasis. In recent years, TD has shown definite benefits in the cardio-cerebrovascular system, but its specific mechanism is not very clear. The purpose of this study was to explore the mechanism by which TD affects cerebral ischaemia/reperfusion (I/R) injury and to optimize the mixture ratio. The affected metabolic pathways in rat brain tissues after I/R were analysed by network pharmacology and verified with animal pharmacological experiments. TD had a certain therapeutic effect on cerebral I/R injury. TD with a TR:DH ratio of 1:1 had the best therapeutic effect. Metabolic pathway analysis showed that the protective mechanism of TD against I/R injury involves mainly regulation of brain tissue ADORA2A protein levels and action on the arachidonic acid (AA) pathway. Conclusion: TD can ameliorate cerebral I/R injury by regulating ADORA2A degradation in the AA metabolic pathway to attenuate AA metabolic dysfunction and the inflammatory response. [Display omitted]