BMP‐15 activity on in vitro development of collared peccary (Pecari tajacu Linnaeus, 1758) preantral follicles

This study investigated the effects of BMP‐15 on the in vitro development of preantral follicles of collared peccaries. Ovarian fragments were cultured for 1 or 6 days in Tissue Culture Medium 199 (TCM199+) supplemented with BMP‐15 at rates of 0, 1, 25 or 50 ng/ml. The fragments were analysed histol...

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Bibliographic Details
Published in:Reproduction in domestic animals 2020-08, Vol.55 (8), p.958-964
Main Authors: Gomes, Henrique A. N., Campos, Lívia B., Praxedes, Érica C. G., Oliveira, Moacir F., Pereira, Alexsandra F., Silva, Alexandre R., Saraiva, Márcia V. A.
Format: Article
Language:English
Subjects:
Cell culture
Cell proliferation
Follicles
follicular activation
folliculogenesis
Fragments
Gametocytes
Granulosa cells
Morphology
Nucleoli
Pecari tajacu
TGFβ family
Tissue culture
wild mammals
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Summary:This study investigated the effects of BMP‐15 on the in vitro development of preantral follicles of collared peccaries. Ovarian fragments were cultured for 1 or 6 days in Tissue Culture Medium 199 (TCM199+) supplemented with BMP‐15 at rates of 0, 1, 25 or 50 ng/ml. The fragments were analysed histologically by evaluating follicular morphology, activation and growth as well as the potential for proliferation of granulosa cells. Our results show the addition of 25 ng/ml BMP‐15 in the medium provided the greatest percentage of normal follicles (79.67% ± 0.69) when compared to other treatments (p  .05). Moreover, 25 and 50 ng/ml of BMP‐15 promoted follicular activation. BMP‐15 supplements did not affect oocyte and follicular growth. All concentrations of BMP‐15 increased the number of nucleolus organizer regions (NORs) after 1 day of culture when compared to fresh fragments or the control samples (p  .05). In summary, the addition of 25 ng/ml BMP‐15 to the culture medium of collared peccary preantral follicles maintained a high number of morphologically healthy follicles and stimulated the activation of primordial follicles after 6 days in culture.
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.13735