BMP‐15 activity on in vitro development of collared peccary (Pecari tajacu Linnaeus, 1758) preantral follicles

• Published in: Reproduction in domestic animals 2020-08, Vol.55 (8), p.958-964
• Main Authors: Gomes, Henrique A. N., Campos, Lívia B., Praxedes, Érica C. G., Oliveira, Moacir F., Pereira, Alexsandra F., Silva, Alexandre R., Saraiva, Márcia V. A.
• Format: Article
• Language: English
• Subjects: Cell culture; Cell proliferation; Follicles; follicular activation; folliculogenesis; Fragments; Gametocytes; Granulosa cells; Morphology; Nucleoli; Pecari tajacu; TGFβ family; Tissue culture; wild mammals
• ISSN: 0936-6768; 1439-0531
• DOI: 10.1111/rda.13735

This study investigated the effects of BMP‐15 on the in vitro development of preantral follicles of collared peccaries. Ovarian fragments were cultured for 1 or 6 days in Tissue Culture Medium 199 (TCM199+) supplemented with BMP‐15 at rates of 0, 1, 25 or 50 ng/ml. The fragments were analysed histologically by evaluating follicular morphology, activation and growth as well as the potential for proliferation of granulosa cells. Our results show the addition of 25 ng/ml BMP‐15 in the medium provided the greatest percentage of normal follicles (79.67% ± 0.69) when compared to other treatments (p  .05). Moreover, 25 and 50 ng/ml of BMP‐15 promoted follicular activation. BMP‐15 supplements did not affect oocyte and follicular growth. All concentrations of BMP‐15 increased the number of nucleolus organizer regions (NORs) after 1 day of culture when compared to fresh fragments or the control samples (p  .05). In summary, the addition of 25 ng/ml BMP‐15 to the culture medium of collared peccary preantral follicles maintained a high number of morphologically healthy follicles and stimulated the activation of primordial follicles after 6 days in culture.