Evaluation of Copan FecalSwab™ preserved stool specimens with the BD MAX™ Enteric Bacterial Panel and the BD MAX™ Extended Enteric Bacterial Panel

• Published in: Diagnostic microbiology and infectious disease 2020-08, Vol.97 (4), p.115055-115055, Article 115055
• Main Authors: Rojas, Hanen Fernandez, Lima, Amorce, Kubasek, Carly, Gostnell, Alicia, Silbert, Suzane
• Format: Article
• Language: English
• Subjects: Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; BD MAX; Copan FecalSwab; Culture Media; Enteric pathogens; Feces - microbiology; Gastroenteritis - diagnosis; Gastroenteritis - microbiology; Gastrointestinal Microbiome - genetics; Gastrointestinal panel; Humans; Microbiological Techniques - methods; Molecular Diagnostic Techniques; Sensitivity and Specificity; Specimen Handling - methods
• ISSN: 0732-8893; 1879-0070
• DOI: 10.1016/j.diagmicrobio.2020.115055

The objectives of this study were to assess the ideal volume of Copan FecalSwab™ (FS) preserved stool sample to use with the BD MAX™ Enteric Bacterial Panel and the Extended Enteric Bacterial Panel (BDM GIP) and to compare the performance of FS to the recommended Meridian Para-Pak Cary-Blair medium (PP) for the BDM GIP. Three different input volumes (10, 25, and 50 μL) of stool inoculated with American Type Culture Collection strains representing the targets detected by BDM GIP were tested. Additionally, 144 unpreserved stool samples submitted for gastrointestinal (GI) testing were transferred to PP and FS media and tested by the BDM GIP using 10 μL of PP and 50 μL of FS media. A 100% agreement was observed between PP and FS results. The performance of 50 μL of FS stool preserved sample was equivalent to 10 μL of traditional Cary-Blair PP preserved specimens for GI pathogens detection using the BDM GIP.