NOD Mice Recapitulate the Cardiac Disturbances Observed in Type 1 Diabetes

This work aimed at testing the hypothesis that NOD/ShiLtJ mice (NOD) recapitulate the cardiac disturbances observed on type 1 diabetes (T1D). NOD mice were studied 4 weeks after the onset of hyperglycemia, and NOR/Lt mice matched as control. Cardiac function was evaluated by echocardiography and ele...

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Published in:Journal of cardiovascular translational research 2021-04, Vol.14 (2), p.271-282
Main Authors: Schleier, Ygor, Moreno-Loaiza, Oscar, López Alarcón, Maria Micaela, Lopes Martins, Eduarda Gabrielle, Braga, Bruno Cabral, Ramos, Isalira Peroba, Galina, Antonio, Medei, Emiliano Horacio
Format: Article
Language:English
Subjects:
Action Potentials
Animals
Arrhythmias, Cardiac - etiology
Arrhythmias, Cardiac - metabolism
Arrhythmias, Cardiac - physiopathology
Biomedical Engineering and Bioengineering
Biomedicine
Blood Glucose - metabolism
Calcium Signaling
Cardiology
Diabetes Mellitus, Type 1 - blood
Diabetes Mellitus, Type 1 - complications
Disease Models, Animal
Heart Conduction System - metabolism
Heart Conduction System - physiopathology
Heart Rate
Human Genetics
Kinetics
Medicine
Medicine & Public Health
Mice
Mice, Inbred NOD
Mitochondria, Heart - metabolism
Myocytes, Cardiac - metabolism
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Summary:This work aimed at testing the hypothesis that NOD/ShiLtJ mice (NOD) recapitulate the cardiac disturbances observed on type 1 diabetes (T1D). NOD mice were studied 4 weeks after the onset of hyperglycemia, and NOR/Lt mice matched as control. Cardiac function was evaluated by echocardiography and electrocardiography (ECG). Action potentials (AP) and Ca 2+ transients were evaluated at whole heart level. Heart mitochondrial function was evaluated by high-resolution respirometry and H 2 O 2 release. NOD mice presented a reduction in hearth weight. Mitochondrial oxygen fluxes and H 2 O 2 release were similar between NOD and NOR mice. ECG revealed a QJ interval prolongation in NOD mice. Furthermore, AP duration at 30% of repolarization was increased, and it depicted slower Ca 2+ transient kinetics. NOD mice presented greater number/severity of ventricular arrhythmias both in vivo and in vitro. In conclusion, NOD mice evoked cardiac electrical and calcium handling disturbances similar to the observed in T1D. Graphical Abstract .
ISSN:1937-5387
1937-5395
DOI:10.1007/s12265-020-10039-y