An approach to rescue the fertility of stallions with a high level of hemospermia

A high amount of blood and not the mere presence of blood in equine semen impacts fertility. The aim of this study was to develop an approach to rescue the fertility of stallions with high hemospermia levels. Semen from 15 stallions was divided into four experimental groups: (a) Control—pure raw sem...

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Bibliographic Details
Published in:Reproduction in domestic animals 2020-09, Vol.55 (9), p.1258-1262
Main Authors: Andrade Junior, Luiz Roberto P., Segabinazzi, Lorenzo G. T. M., Oliveira, Sidnei N., Dell'Aqua, José Antonio, Papa, Frederico O.
Format: Article
Language:English
Subjects:
Blood
Dilution
equine
Fertility
Horses
Kinetics
Lipid peroxidation
Lipids
Milk
Motility
Ovulation
penis
Peroxidation
Pregnancy
Semen
Sperm
Superoxide
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Summary:A high amount of blood and not the mere presence of blood in equine semen impacts fertility. The aim of this study was to develop an approach to rescue the fertility of stallions with high hemospermia levels. Semen from 15 stallions was divided into four experimental groups: (a) Control—pure raw semen, (b) WB50—50% (v/v) whole blood added into semen, (c) E1—WB50 extended in a 1:1 (v/v) ratio with milk‐based extender and (d) E2—WB50 extended in a 2:1 ratio with milk‐based extender. Sperm kinetics, plasma membrane integrity (PMI), lipid peroxidation (PER) and intracellular superoxide (O2) production were immediately evaluated. Four cycles of 20 mares were randomly assigned to the experimental groups. Mares were bred with an insemination dose of 1 billion total sperm and pregnancy was diagnosed 14 days after ovulation. Sperm kinetics could not be evaluated in the WB50 samples. Total motility was lower (p 
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.13717