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Contribution of autochthonous yeasts with probiotic potential to the aroma profile of fermented Guajillo pepper sauce

BACKGROUND Three yeast strains with probiotic potential, Hanseniaspora opuntiae, Pichia kudriavzevii, and Wickerhamomyces anomalus were inoculated in the fermentation of Guajillo chilli pepper (Capsicum annuum L.) sauce, and the different aroma profiles were investigated. Using headspace solid‐phase...

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Published in:Journal of the science of food and agriculture 2020-10, Vol.100 (13), p.4940-4949
Main Authors: Lara‐Hidalgo, Carlos, Belloch, Carmela, Dorantes‐Alvarez, Lidia, Flores, Mónica
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creator Lara‐Hidalgo, Carlos
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Dorantes‐Alvarez, Lidia
Flores, Mónica
description BACKGROUND Three yeast strains with probiotic potential, Hanseniaspora opuntiae, Pichia kudriavzevii, and Wickerhamomyces anomalus were inoculated in the fermentation of Guajillo chilli pepper (Capsicum annuum L.) sauce, and the different aroma profiles were investigated. Using headspace solid‐phase microextraction‐gas chromatography‐mass spectrometry (HS‐SPME‐GC‐MS) analysis and gas chromatography‐olfactometry (GCO), flavour compound production was evaluated during the fermentation of the Guajillo chilli pepper sauces. RESULTS A total of 78 volatile compounds were identified during the yeast fermentation of the sauce. Most aldehydes and terpenes detected were present at the beginning of the fermentation, indicating a Guajillo chilli pepper origin. Among the 34 active aroma compounds detected by GCO, propanoic acid (cheesy), 3‐methylbutanoic acid (sharp, cheese), ethyl 2‐methylbutanoate (fruity), and 6‐methyl‐5‐hepten‐2‐one (strong, citrus) were identified as key aroma contributors produced by the inoculation of the yeasts. A different aroma profile was produced by probiotic yeast. Hanseniaspora opuntiae produced an aroma profile with herbal and green notes based on high production of aldehydes, ketones, and acetic acid. Pichia kudriavzevii and W. anomalus produced fruity, green‐herbal, and cheesy notes based on ester compounds, alcohol and branched‐chain acids production although, the production of propanoic acid by W. anomalus increased the cheesy character in the sauces. CONCLUSION The aroma profile of fermented chilli pepper sauces depends not only on the chili pepper varieties used but also on the fermentation process as a source of aroma compounds. The use of probiotic yeast can be used to improve and diversify the aroma profile of fermented chilli pepper sauces. © 2020 Society of Chemical Industry
doi_str_mv 10.1002/jsfa.10556
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Using headspace solid‐phase microextraction‐gas chromatography‐mass spectrometry (HS‐SPME‐GC‐MS) analysis and gas chromatography‐olfactometry (GCO), flavour compound production was evaluated during the fermentation of the Guajillo chilli pepper sauces. RESULTS A total of 78 volatile compounds were identified during the yeast fermentation of the sauce. Most aldehydes and terpenes detected were present at the beginning of the fermentation, indicating a Guajillo chilli pepper origin. Among the 34 active aroma compounds detected by GCO, propanoic acid (cheesy), 3‐methylbutanoic acid (sharp, cheese), ethyl 2‐methylbutanoate (fruity), and 6‐methyl‐5‐hepten‐2‐one (strong, citrus) were identified as key aroma contributors produced by the inoculation of the yeasts. A different aroma profile was produced by probiotic yeast. Hanseniaspora opuntiae produced an aroma profile with herbal and green notes based on high production of aldehydes, ketones, and acetic acid. Pichia kudriavzevii and W. anomalus produced fruity, green‐herbal, and cheesy notes based on ester compounds, alcohol and branched‐chain acids production although, the production of propanoic acid by W. anomalus increased the cheesy character in the sauces. CONCLUSION The aroma profile of fermented chilli pepper sauces depends not only on the chili pepper varieties used but also on the fermentation process as a source of aroma compounds. The use of probiotic yeast can be used to improve and diversify the aroma profile of fermented chilli pepper sauces. © 2020 Society of Chemical Industry</description><identifier>ISSN: 0022-5142</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/jsfa.10556</identifier><identifier>PMID: 32474932</identifier><language>eng</language><publisher>Chichester, UK: John Wiley &amp; Sons, Ltd</publisher><subject>Acetic acid ; Acids ; Aldehydes ; aroma ; Aroma compounds ; Capsicum - chemistry ; Capsicum - microbiology ; Chain branching ; chili pepper ; Chromatography ; Fermentation ; Fermented Foods and Beverages - analysis ; Fermented Foods and Beverages - microbiology ; Flavor compounds ; Flavoring Agents - chemistry ; Flavoring Agents - metabolism ; Flavors ; Gas chromatography ; Gas Chromatography-Mass Spectrometry ; Hanseniaspora - metabolism ; Hanseniaspora opuntiae ; Headspace ; Humans ; Inoculation ; Ketones ; Mass spectrometry ; Mass spectroscopy ; Odorants - analysis ; Olfactometers ; Olfactometry ; Peppers ; Pichia - metabolism ; Pichia kudriavzevii ; Probiotics ; Probiotics - analysis ; Propionic acid ; Saccharomycetales - metabolism ; Sauces ; Solid phase methods ; Taste ; Terpenes ; Vegetable Products - analysis ; Vegetable Products - microbiology ; Volatile compounds ; Volatile Organic Compounds - chemistry ; Volatile Organic Compounds - metabolism ; Wickerhamomyces anomalus ; Yeast ; Yeasts</subject><ispartof>Journal of the science of food and agriculture, 2020-10, Vol.100 (13), p.4940-4949</ispartof><rights>2020 Society of Chemical Industry</rights><rights>2020 Society of Chemical Industry.</rights><rights>Copyright © 2020 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3086-9c8243666d2e30cafcd656dce8f837ac1e464bef9697185704ca9353ef165b203</citedby><cites>FETCH-LOGICAL-c3086-9c8243666d2e30cafcd656dce8f837ac1e464bef9697185704ca9353ef165b203</cites><orcidid>0000-0002-4228-9899</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32474932$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lara‐Hidalgo, Carlos</creatorcontrib><creatorcontrib>Belloch, Carmela</creatorcontrib><creatorcontrib>Dorantes‐Alvarez, Lidia</creatorcontrib><creatorcontrib>Flores, Mónica</creatorcontrib><title>Contribution of autochthonous yeasts with probiotic potential to the aroma profile of fermented Guajillo pepper sauce</title><title>Journal of the science of food and agriculture</title><addtitle>J Sci Food Agric</addtitle><description>BACKGROUND Three yeast strains with probiotic potential, Hanseniaspora opuntiae, Pichia kudriavzevii, and Wickerhamomyces anomalus were inoculated in the fermentation of Guajillo chilli pepper (Capsicum annuum L.) sauce, and the different aroma profiles were investigated. Using headspace solid‐phase microextraction‐gas chromatography‐mass spectrometry (HS‐SPME‐GC‐MS) analysis and gas chromatography‐olfactometry (GCO), flavour compound production was evaluated during the fermentation of the Guajillo chilli pepper sauces. RESULTS A total of 78 volatile compounds were identified during the yeast fermentation of the sauce. Most aldehydes and terpenes detected were present at the beginning of the fermentation, indicating a Guajillo chilli pepper origin. Among the 34 active aroma compounds detected by GCO, propanoic acid (cheesy), 3‐methylbutanoic acid (sharp, cheese), ethyl 2‐methylbutanoate (fruity), and 6‐methyl‐5‐hepten‐2‐one (strong, citrus) were identified as key aroma contributors produced by the inoculation of the yeasts. A different aroma profile was produced by probiotic yeast. Hanseniaspora opuntiae produced an aroma profile with herbal and green notes based on high production of aldehydes, ketones, and acetic acid. Pichia kudriavzevii and W. anomalus produced fruity, green‐herbal, and cheesy notes based on ester compounds, alcohol and branched‐chain acids production although, the production of propanoic acid by W. anomalus increased the cheesy character in the sauces. CONCLUSION The aroma profile of fermented chilli pepper sauces depends not only on the chili pepper varieties used but also on the fermentation process as a source of aroma compounds. 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Using headspace solid‐phase microextraction‐gas chromatography‐mass spectrometry (HS‐SPME‐GC‐MS) analysis and gas chromatography‐olfactometry (GCO), flavour compound production was evaluated during the fermentation of the Guajillo chilli pepper sauces. RESULTS A total of 78 volatile compounds were identified during the yeast fermentation of the sauce. Most aldehydes and terpenes detected were present at the beginning of the fermentation, indicating a Guajillo chilli pepper origin. Among the 34 active aroma compounds detected by GCO, propanoic acid (cheesy), 3‐methylbutanoic acid (sharp, cheese), ethyl 2‐methylbutanoate (fruity), and 6‐methyl‐5‐hepten‐2‐one (strong, citrus) were identified as key aroma contributors produced by the inoculation of the yeasts. A different aroma profile was produced by probiotic yeast. Hanseniaspora opuntiae produced an aroma profile with herbal and green notes based on high production of aldehydes, ketones, and acetic acid. Pichia kudriavzevii and W. anomalus produced fruity, green‐herbal, and cheesy notes based on ester compounds, alcohol and branched‐chain acids production although, the production of propanoic acid by W. anomalus increased the cheesy character in the sauces. CONCLUSION The aroma profile of fermented chilli pepper sauces depends not only on the chili pepper varieties used but also on the fermentation process as a source of aroma compounds. The use of probiotic yeast can be used to improve and diversify the aroma profile of fermented chilli pepper sauces. © 2020 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley &amp; Sons, Ltd</pub><pmid>32474932</pmid><doi>10.1002/jsfa.10556</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-4228-9899</orcidid><oa>free_for_read</oa></addata></record>
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subjects Acetic acid
Acids
Aldehydes
aroma
Aroma compounds
Capsicum - chemistry
Capsicum - microbiology
Chain branching
chili pepper
Chromatography
Fermentation
Fermented Foods and Beverages - analysis
Fermented Foods and Beverages - microbiology
Flavor compounds
Flavoring Agents - chemistry
Flavoring Agents - metabolism
Flavors
Gas chromatography
Gas Chromatography-Mass Spectrometry
Hanseniaspora - metabolism
Hanseniaspora opuntiae
Headspace
Humans
Inoculation
Ketones
Mass spectrometry
Mass spectroscopy
Odorants - analysis
Olfactometers
Olfactometry
Peppers
Pichia - metabolism
Pichia kudriavzevii
Probiotics
Probiotics - analysis
Propionic acid
Saccharomycetales - metabolism
Sauces
Solid phase methods
Taste
Terpenes
Vegetable Products - analysis
Vegetable Products - microbiology
Volatile compounds
Volatile Organic Compounds - chemistry
Volatile Organic Compounds - metabolism
Wickerhamomyces anomalus
Yeast
Yeasts
title Contribution of autochthonous yeasts with probiotic potential to the aroma profile of fermented Guajillo pepper sauce
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